Attachment and proliferation of human osteoblast-like cells (MG-63) on laser-ablated titanium implant material
Demand is increasing for shortening the long (3-6months) osseointegration period to rehabilitate patients' damaged chewing apparatus in as short a time as possible. For dental implants, as for biomaterials in general, the bio- and osseointegration processes can be controlled at molecular and c...
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| Dokumentumtípus: | Cikk |
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Elsevier BV
2013
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| Sorozat: | MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
33 No. 7 |
| doi: | 10.1016/j.msec.2013.06.020 |
| mtmt: | 2377533 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/9367 |
| Tartalmi kivonat: | Demand is increasing for shortening the long (3-6months) osseointegration period to rehabilitate patients' damaged chewing apparatus in as short a time as possible. For dental implants, as for biomaterials in general, the bio- and osseointegration processes can be controlled at molecular and cellular levels by modification of the implant surface. One of the most promising of such surface modifications is laser ablation, as demonstrated by our previous results [46]. Commercially pure (CP4) sand-blasted, acid-etched titanium disks (Denti(R) System Ltd., Hungary) were irradiated with a KrF excimer laser (248nm, fluence 0.4J/cm(2), FWHM 18ns, 2000 pulses), or with a Nd:YAG laser (532nm, 1.3J/cm(2), 10ns, 200 pulses) then examined by SEM, AFM, and XPS. In vitro attachment (24h) and proliferation (72h) of MG-63 osteoblast cells were investigated via dimethylthiazol-diphenyl tetrazolium bromide (MTT), alamarBlue (AB) assays alkaline phosphatase quantification (ALP) and SEM. SEM and AFM revealed significant changes in morphology and roughness. XPS confirmed the presence of TiO2 on each sample; after Nd:YAG treatment a reduced state of Ti (Ti(3+)) was also observed. MTT, AB and ALP measurements detected an increase in the number of cells between the 24- and 72hour observations; however, laser treatment did not affect cell attachment and proliferation significantly. |
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| Terjedelem/Fizikai jellemzők: | 4251-4259 |
| ISSN: | 0928-4931 |