Heat-stable Camel Milk-base Culture Medium Efficiency to Improve Microbiological Analysis of Raw Camel Milk
The development of an optimal culture medium for microbiological analysis is a significant advancement in the field. The conventional PCA for RAMF enumeration may not accurately assess raw camel milk’s microflora, potentially compromising food safety evaluations. Our study addresses this crucial iss...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2025
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| Sorozat: | ASIAN JOURNAL OF DAIRY AND FOOD RESEARCH
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| Tárgyszavak: | |
| doi: | 10.18805/ajdfr.DRF-528 |
| mtmt: | 36225204 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/37173 |
| Tartalmi kivonat: | The development of an optimal culture medium for microbiological analysis is a significant advancement in the field. The conventional PCA for RAMF enumeration may not accurately assess raw camel milk’s microflora, potentially compromising food safety evaluations. Our study addresses this crucial issue. We developed CaM-PCA medium using sterilized whole camel milk and compared it with standard PCA using twenty-three raw milk samples. Enumeration results were analyzed using Mann-Whitney test and exclusive isolates were identified through 16S rDNA sequencing. CaM-PCA yielded significantly higher RAMF counts (8.50±0.45 log10 CFU ml-1) than PCA (8.20±0.60 log10 CFU ml-1), P=0.048. Three isolates exclusive to CaM-PCA were identified as Metabacillus sp., Hafnia alvei and Enterobacter hormaechi subsp. hoffmannii, demonstrating CaM-PCA’s enhanced ability to detect spoilage and opportunistic pathogens in raw camel milk. |
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| ISSN: | 0971-4456 |