Hplc determination of enantiomeric purity of protected amino acid derivatives used in peptide synthesis
An improved RP-HPLC method on ODS-Hypersil column with precolumn derivatization with Marfey's reagent were used to monitor racemization in N-, C- and/or side-chain protected amino acid derivatives by separation of a series of new diastereoisomeric Marfey's compounds. Chromatographic sample...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
1994
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| Sorozat: | JOURNAL OF LIQUID CHROMATOGRAPHY
17 No. 13 |
| Tárgyszavak: | |
| doi: | 10.1080/10826079408013498 |
| mtmt: | 1880327 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/31995 |
| Tartalmi kivonat: | An improved RP-HPLC method on ODS-Hypersil column with precolumn derivatization with Marfey's reagent were used to monitor racemization in N-, C- and/or side-chain protected amino acid derivatives by separation of a series of new diastereoisomeric Marfey's compounds. Chromatographic samples were obtained by partial deprotection of different starting materials. In a simple two-step procedure (deprotection and derivatization) the compounds of amino acids formed stable diastereomeric derivatives having facile resolutions. |
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| Terjedelem/Fizikai jellemzők: | 2759-2775 |
| ISSN: | 0148-3919 |