Production and purification of low calcium response protein h of chlamydophila pneumoniae
Chlamydophila pneumoniae possesses a type III secretion system (TTSS), which allows the bacteria to secrete effector molecules into the inclusion membrane and into the cytosol of the host cell. Low calcium response protein H (LcrH), as a part of the TTSS, is a chaperone protein expressed from the mi...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2009
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| Sorozat: | ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA
56 No. 4 |
| Tárgyszavak: | |
| doi: | 10.1556/AMicr.56.2009.4.8 |
| mtmt: | 1347852 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/28604 |
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| 024 | 7 | |a 10.1556/AMicr.56.2009.4.8 |2 doi | |
| 024 | 7 | |a 1347852 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a Angol | ||
| 100 | 1 | |a Faludi Ildikó | |
| 245 | 1 | 0 | |a Production and purification of low calcium response protein h of chlamydophila pneumoniae |h [elektronikus dokumentum] / |c Faludi Ildikó |
| 260 | |c 2009 | ||
| 300 | |a 389-397 | ||
| 490 | 0 | |a ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA |v 56 No. 4 | |
| 520 | 3 | |a Chlamydophila pneumoniae possesses a type III secretion system (TTSS), which allows the bacteria to secrete effector molecules into the inclusion membrane and into the cytosol of the host cell. Low calcium response protein H (LcrH), as a part of the TTSS, is a chaperone protein expressed from the middle to late stages of the chlamydial developmental cycle. Gene of LcrH (CPn0811) in a 6His-tagged form was cloned from C. pneumoniae CWL029, expressed and purified from Escherichia coli using the HIS-select TALON CellThru Resin. The purity was checked with mass spectrometry. The samples were used for immunization of BALB/c mice. The inducible E. coli clone, which over-expresses the chlamydial LcrH, permits the study of the biological properties of this protein. | |
| 650 | 4 | |a Biológiai tudományok | |
| 650 | 4 | |a Általános orvostudomány | |
| 700 | 0 | 1 | |a Csanádi Ágnes |e aut |
| 700 | 0 | 1 | |a Szabó Ágnes Míra |e aut |
| 700 | 0 | 1 | |a Burián Katalin |e aut |
| 700 | 0 | 1 | |a Endrész Valéria |e aut |
| 700 | 0 | 1 | |a Miczák András |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/28604/1/1347852_030-article-p389.pdf |z Dokumentum-elérés |