Production and purification of low calcium response protein h of chlamydophila pneumoniae
Chlamydophila pneumoniae possesses a type III secretion system (TTSS), which allows the bacteria to secrete effector molecules into the inclusion membrane and into the cytosol of the host cell. Low calcium response protein H (LcrH), as a part of the TTSS, is a chaperone protein expressed from the mi...
Elmentve itt :
| Szerzők: | |
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2009
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| Sorozat: | ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA
56 No. 4 |
| Tárgyszavak: | |
| doi: | 10.1556/AMicr.56.2009.4.8 |
| mtmt: | 1347852 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/28604 |
| Tartalmi kivonat: | Chlamydophila pneumoniae possesses a type III secretion system (TTSS), which allows the bacteria to secrete effector molecules into the inclusion membrane and into the cytosol of the host cell. Low calcium response protein H (LcrH), as a part of the TTSS, is a chaperone protein expressed from the middle to late stages of the chlamydial developmental cycle. Gene of LcrH (CPn0811) in a 6His-tagged form was cloned from C. pneumoniae CWL029, expressed and purified from Escherichia coli using the HIS-select TALON CellThru Resin. The purity was checked with mass spectrometry. The samples were used for immunization of BALB/c mice. The inducible E. coli clone, which over-expresses the chlamydial LcrH, permits the study of the biological properties of this protein. |
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| Terjedelem/Fizikai jellemzők: | 389-397 |
| ISSN: | 1217-8950 |