Capacity and kinetics of light-induced cytochrome oxidation in intact cells of photosynthetic bacteria
Light-induced oxidation of the reaction center dimer and periplasmic cytochromes was detected by fast kinetic difference absorption changes in intact cells of wild type and cytochrome mutants ( cycA , cytC4 and pufC ) of Rubrivivax gelatinosus and Rhodobacter sphaeroides . Constant illumination from...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
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2022
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| Sorozat: | SCIENTIFIC REPORTS
12 No. 1 |
| Tárgyszavak: | |
| doi: | 10.1038/s41598-022-18399-y |
| mtmt: | 33073139 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/27163 |
| LEADER | 02289nab a2200253 i 4500 | ||
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| 005 | 20230512095802.0 | ||
| 008 | 230512s2022 hu o 0|| Angol d | ||
| 022 | |a 2045-2322 | ||
| 024 | 7 | |a 10.1038/s41598-022-18399-y |2 doi | |
| 024 | 7 | |a 33073139 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a Angol | ||
| 100 | 1 | |a Kis Mariann | |
| 245 | 1 | 0 | |a Capacity and kinetics of light-induced cytochrome oxidation in intact cells of photosynthetic bacteria |h [elektronikus dokumentum] / |c Kis Mariann |
| 260 | |c 2022 | ||
| 300 | |a 14 | ||
| 490 | 0 | |a SCIENTIFIC REPORTS |v 12 No. 1 | |
| 520 | 3 | |a Light-induced oxidation of the reaction center dimer and periplasmic cytochromes was detected by fast kinetic difference absorption changes in intact cells of wild type and cytochrome mutants ( cycA , cytC4 and pufC ) of Rubrivivax gelatinosus and Rhodobacter sphaeroides . Constant illumination from a laser diode or trains of saturating flashes enabled the kinetic separation of acceptor and donor redox processes, and the electron contribution from the cyt bc 1 complex via periplasmic cytochromes. Under continuous excitation, concentrations of oxidized cytochromes increased in three phases where light intensity, electron transfer rate and the number of reduced cytochromes were the rate liming steps, respectively. By choosing suitable flash timing, gradual steps of cytochrome oxidation in whole cells were observed; each successive flash resulted in a smaller, damped oxidation. We attribute this damping to lowered availability of reduced cytochromes resulting from both exchange (unbinding/binding) of the cytochromes and electron transfer at the reaction center interface since a similar effect is observed upon deletion of genes encoding periplasmic cytochromes. In addition, we present a simple model to calculate the damping effect; application of this method may contribute to understanding the function of the diverse range of c-type cytochromes in the electron transport chains of anaerobic phototrophic bacteria. | |
| 650 | 4 | |a Fizikai tudományok | |
| 650 | 4 | |a Biológiai tudományok | |
| 700 | 0 | 1 | |a Smart James L. |e aut |
| 700 | 0 | 1 | |a Maróti Péter |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/27163/1/ScientificReports202208.pdf |z Dokumentum-elérés |