Microcirculatory consequences of osteoporosis
Introduction. The etiology of senile osteoporosis syndrome is multifactorial, but the reduced estrogen levels in peri-menopausal women are clearly associated with an accelerated bone loss. In our experimental studies, the consequences of osteoporosis and chronic estrogen supplementation were investi...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2010
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| Sorozat: | TIMISOARA MEDICAL JOURNAL
60 No. Suppl. 2 |
| Tárgyszavak: | |
| mtmt: | 1895796 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/24946 |
| Tartalmi kivonat: | Introduction. The etiology of senile osteoporosis syndrome is multifactorial, but the reduced estrogen levels in peri-menopausal women are clearly associated with an accelerated bone loss. In our experimental studies, the consequences of osteoporosis and chronic estrogen supplementation were investigated on the periosteal microcirculatory inflammatory reactions and estrogen receptor expressions. Material and method. Six months after the initiation of estrogen replacement therapy with 17β-estradiol (E2, 20 µg-1kg-1day-1) in ovariectomized (OVX) Sprague-Dawley rats, the microcirculatory inflammatory consequences of 60-min total hindlimb ischemia followed by 180-min reperfusion were examined. Leukocyte estrogen receptor expression changes were also assessed 1 month after OVX plus E2 treatment. Results. Apart from reversing the osteopenic effects of OVX, E2 treatment significantly ameliorated the ischemia-reperfusion-induced elevation of neutrophil leukocyte adherence to the postcapillary venules of the periosteum (visualized by means of intravital fluorescence microscopy). This effect was not associated with a reduction of CD11b expression of the neutrophil leukocytes (FACS analysis). OVX caused a moderate decrease in leukocyte estrogen receptor (ER)-beta protein expression which tended to be reversed by E2. Leukocyte ER-alpha and ER-beta mRNA levels (RT-PCR) and the ER-alpha protein expression (Western blotting) were below the detection limit. Conclusions. Chronic estrogen supplementation efficiently ameliorates osteoporosis and the inflammatory microcirculatory consequences of transient limb ischemia. It appears that this effect is not mediated by leukocyte CD11b, or estrogen receptor expression changes. |
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| Terjedelem/Fizikai jellemzők: | 100-106 |
| ISSN: | 1583-5251 |