Characterization and functional analysis of zinc trafficking in the human fungal pathogen Candida parapsilosis

The zinc restriction and zinc toxicity are part of host defence, called nutritional immunity. The crucial role of zinc homeostasis in microbial survival within a host is established, but little is known about these processes in the opportunistic human fungal pathogen Candida parapsilosis. Our in sil...

Teljes leírás

Elmentve itt :
Bibliográfiai részletek
Szerzők: Takács Tamás
Németh Tibor Mihály
Bohner Flóra
Vágvölgyi Csaba
Jankovics Ferenc
Wilson Duncan
Gácser Attila
Dokumentumtípus: Cikk
Megjelent: 2022
Sorozat:OPEN BIOLOGY 12 No. 7
Tárgyszavak:
doi:10.1098/rsob.220077

mtmt:33025619
Online Access:http://publicatio.bibl.u-szeged.hu/24833
Leíró adatok
Tartalmi kivonat:The zinc restriction and zinc toxicity are part of host defence, called nutritional immunity. The crucial role of zinc homeostasis in microbial survival within a host is established, but little is known about these processes in the opportunistic human fungal pathogen Candida parapsilosis. Our in silico predictions suggested the presence of at least six potential zinc transporters (ZnTs) in C. parapsilosis-orthologues of ZRC1, ZRT3 and ZRT101-but an orthologue of PRA1 zincophore was not found. In addition, we detected a species-specific gene expansion of the novel ZnT ZRT2, as we identified three orthologue genes in the genome of C. parapsilosis. Based on predictions, we created homozygous mutant strains of the potential ZnTs and characterized them. Despite the apparent gene expansion of ZRT2 in C. parapsilosis, only CpZRT21 was essential for growth in a zinc-depleted acidic environment, in addition we found that CpZrc1 is essential for zinc detoxification and also protects the fungi against the elimination of murine macrophages. Significantly, we demonstrated that C. parapsilosis forms zincosomes in a Zrc1-independent manner and zinc detoxification is mediated by the vacuolar importer CpZrc1. Our study defines the functions of C. parapsilosis ZnTs, including a species-specific survival and zinc detoxification system.
Terjedelem/Fizikai jellemzők:Terjedelem: 16 p.-Azonosító: 220077
ISSN:2046-2441