Phenotypic plasticity of melanocytes derived from human adult skin

We previously described a novel in vitro culture technique for dedifferentiated human adult skin melanocytes. Melanocytes cultured in a defined, cholera toxin and PMA free medium became bipolar, unpigmented, and highly proliferative. Furthermore, TRP-1 and c-Kit expression disappeared and EGFR recep...

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Bibliographic Details
Main Authors: Vidács Dániel László
Veréb Zoltán
Bozó Renáta
Flink Lili Borbála
Polyánka Hilda
Németh István Balázs
Póliska Szilárd
Papp Benjamin Tamás
Gáspár Róbert
Mirdamadi Seyedmohsen
Manczinger Máté
Kemény Lajos
Csörgő Sándorné Bata Zsuzsanna
Format: Article
Published: 2022
Series:PIGMENT CELL & MELANOMA RESEARCH 35 No. 1
Subjects:
doi:10.1111/pcmr.13012

mtmt:32173395
Online Access:http://publicatio.bibl.u-szeged.hu/22315
Description
Summary:We previously described a novel in vitro culture technique for dedifferentiated human adult skin melanocytes. Melanocytes cultured in a defined, cholera toxin and PMA free medium became bipolar, unpigmented, and highly proliferative. Furthermore, TRP-1 and c-Kit expression disappeared and EGFR receptor and nestin expression were induced in the cells. Here, we further characterized the phenotype of these dedifferentiated cells and by comparing them to mature pigmented melanocytes we detected crucial steps in their phenotype change. Our data suggest that normal adult melanocytes easily dedifferentiate into pluripotent stem cells given the right environment. This dedifferentiation process described here for normal melanocyte is very similar to what has been described for melanoma cells, indicating that phenotype switching driven by environmental factors is a general characteristic of melanocytes that can occur independent of malignant transformation.
Physical Description:38-51
ISSN:1755-1471