High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress
Immunoglobulin G (IgG) glycosylation corresponds well with immune system changes, so it can potentially be used as a biomarker for the consequences of chronic stress such as low-grade inflammation and enhanced immunosenescence in older animals. Here we present a high-throughput glycoproteomic workfl...
Elmentve itt :
| Szerzők: | |
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2021
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| Sorozat: | JOURNAL OF PROTEOMICS
245 |
| doi: | 10.1016/j.jprot.2021.104293 |
| mtmt: | 32078272 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/21772 |
| LEADER | 02929nab a2200325 i 4500 | ||
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| 024 | 7 | |a 10.1016/j.jprot.2021.104293 |2 doi | |
| 024 | 7 | |a 32078272 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
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| 100 | 1 | |a Habazin Siniša | |
| 245 | 1 | 0 | |a High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress |h [elektronikus dokumentum] / |c Habazin Siniša |
| 260 | |c 2021 | ||
| 300 | |a Terjedelem: 9-Azonosító: 104293 | ||
| 490 | 0 | |a JOURNAL OF PROTEOMICS |v 245 | |
| 520 | 3 | |a Immunoglobulin G (IgG) glycosylation corresponds well with immune system changes, so it can potentially be used as a biomarker for the consequences of chronic stress such as low-grade inflammation and enhanced immunosenescence in older animals. Here we present a high-throughput glycoproteomic workflow, including IgG enrichment, HILIC glycopeptide purification, and nano-LC-MS analysis of tryptic glycopeptides applied for the analysis of rat IgG. A cohort of 80 animals was exposed to seven stressors in a customized chronic stress protocol with blood and tissue sampling in three timepoints. Young female rats experienced an increase in agalactosylated glycoforms on IgG2a and IgG2c accompanied by a decrease in monogalactosylation. Among old females, increased galactosylation was observed in the IgG2b subclass, pointing to an anti-inflammatory activity of IgG. Additionally, IgG Fc N-glycosylation patterns in Sprague Dawley rats were analyzed, quantified, and reported for the first time. Our findings emphasize age-, sex- and subclass-dependent differences in IgG glycosylation related to chronic stress exposure, confirming the relevance of newly developed methods for further research in glycobiology of rodent immune response. SIGNIFICANCE: In this study, we showed that a high-throughput streamlined methodology based on protein L 96-well monolithic plates for efficient rat IgG immunoaffinity enrichment from blood plasma, paired with appropriate tryptic glycopeptide preparation, HILIC-SPE enrichment, and nano-LC-MS methods was suitable for quick processing of large sample sets. We report a subclass-specific profiling and changes in rat IgG Fc galactosylation and adrenal gland immunohistochemistry of male and female animals exposed to a customized chronic stress protocol. | |
| 700 | 0 | 1 | |a Mlinarević Dražen |e aut |
| 700 | 0 | 1 | |a Balog Marta |e aut |
| 700 | 0 | 1 | |a Bardak Ana |e aut |
| 700 | 0 | 1 | |a Gáspár Róbert |e aut |
| 700 | 0 | 1 | |a Szűcs Kálmán Ferenc |e aut |
| 700 | 0 | 1 | |a Vari Sandor G. |e aut |
| 700 | 0 | 1 | |a Vučković Frano |e aut |
| 700 | 0 | 1 | |a Lauc Gordan |e aut |
| 700 | 0 | 1 | |a Novokmet Mislav |e aut |
| 700 | 0 | 1 | |a Heffer Marija |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/21772/1/HabazinetalJProteom2021.pdf |z Dokumentum-elérés |