Esophageal ATP synthase and keratinocyte growth factor gene expression changes after acid and bile-induced mucosal damage
Objective and design: Intramural gene expression changes may be critically involved in tissue damage, defense and repair after esophageal regurgitation. The aims were to characterize the consequences of short-term exposure to luminal bile, acid, or bile mixed with acid on the β-ATPase, keratinocyte...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2005
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| Sorozat: | INFLAMMATION RESEARCH
54 No. 4 |
| doi: | 10.1007/s00011-004-1336-3 |
| mtmt: | 1007352 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/18775 |
| Tartalmi kivonat: | Objective and design: Intramural gene expression changes may be critically involved in tissue damage, defense and repair after esophageal regurgitation. The aims were to characterize the consequences of short-term exposure to luminal bile, acid, or bile mixed with acid on the β-ATPase, keratinocyte growth factor 1 (KGF-1) and KGF receptor (KGF-R) expressions within the mucosa and the muscle layer in a large animal model. Materials and subjects: Esophageal segments of anesthetized dogs were exposed to saline (n = 3), diluted canine bile ( n = 6), hydrochloric acid ( n = 5) or bile + hydrochloric acid ( n = 5), and tissue biopsies were taken at the end of the 180-min observation period. Semiquantitative reverse transcriptase polymerase chain reactions were carried out and the degree of histological damage was evaluated on the 0 - 16-grade Geisinger scoring scale. Results: Acid exposure was followed by a significant decrease in the level of β-ATPase expression in the mucosa, and parallel increases in KGF-1 and KGF- R expression. Corresponding changes in the muscle layer were not significant. Bile alone evoked more severe tissue damage, with significantly decreased β-ATPase levels in both the mucosa and the muscle, whereas the KGF- 1 expression did not change significantly. The bile + acid treatment induced an intermediate state, with significant β-ATPase transcription level decreases in both layers, while the mucosal KGF- 1 expression was lower than that following acid treatment alone. Conclusions: The acid-induced transcriptional level down-regulation of mucosal β-ATPase gene expression in the smooth muscle layer was exacerbated by bile, but the concomitant KGF and KGF- R gene expression changes may indicate the start of a consecutive repair process. |
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| Terjedelem/Fizikai jellemzők: | 152-157 |
| ISSN: | 1023-3830 |