Kupffer cell phagocytosis blockade decreases morbidity in endotoxemic rats with obstructive jaundice
Objective and design: The consequences of Kupffer cell phagocytosis blockade were studied in endotoxemic rats with obstructive jaundice. Material or Subjects: 159 male Wistar rats. Treatment: Obstructive jaundice was induced by bile duct ligation (BDL). Gadolinium chloride (1 mg/100g iv) was given 6...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2002
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| Sorozat: | INFLAMMATION RESEARCH
51 No. 10 |
| doi: | 10.1007/PL00012421 |
| mtmt: | 1320830 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/18765 |
| Tartalmi kivonat: | Objective and design: The consequences of Kupffer cell phagocytosis blockade were studied in endotoxemic rats with obstructive jaundice. Material or Subjects: 159 male Wistar rats. Treatment: Obstructive jaundice was induced by bile duct ligation (BDL). Gadolinium chloride (1 mg/100g iv) was given 6 days after BDL to inhibit Kupffer cell activity and the animals were challenged with 1 mug/g endotoxin 24 h later. Methods: Endotoxin sensitivity, tumor necrosis factor-a and interleukin-6 production were studied, liver and lung injury were assessed by neutrophil infiltration assay, tissue adenosine triphosphate, aspartate aminotransferase, alanine aminotransferase level determinations and histology, respectively. For statistics non-parametric methods were used. Results: BDL sensitized the animals to endotoxin, increased endotoxin-induced tumor necrosis factor-a and interleukin-6 production and reduced ATP contents of the liver and the lung. Kupffer cell blockade significantly increased the resistance against endotoxin, diminished the inflammatory cytokine release and reduced endotoxin-induced tissue injury in BDL animals. Conclusion: Attenuation of Kupffer cell function decreases endotoxin-induced lethality and morbidity in obstructive jaundice. |
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| Terjedelem/Fizikai jellemzők: | 511-518 |
| ISSN: | 1023-3830 |