Nitro-Oleic Acid in Seeds and Differently Developed Seedlings of Brassica napus L.
Similar to animals, it has recently been proven that nitro-fatty acids such as nitro-linolenic acid and nitro-oleic acid (NO2-OA) have relevant physiological roles as signalling molecules also in plants. Although NO2-OA is of great therapeutic importance, its presence in plants as a free fatty acid...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2020
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| Sorozat: | PLANTS
9 No. 3 |
| doi: | 10.3390/plants9030406 |
| mtmt: | 31262237 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/18450 |
| Tartalmi kivonat: | Similar to animals, it has recently been proven that nitro-fatty acids such as nitro-linolenic acid and nitro-oleic acid (NO2-OA) have relevant physiological roles as signalling molecules also in plants. Although NO2-OA is of great therapeutic importance, its presence in plants as a free fatty acid has not been observed so far. Since Brassica napus (oilseed rape) is a crop with high oleic acid content, the abundance of NO2-OA in its tissues can be assumed. Therefore, we quantified NO2-OA in B. napus seeds and differently developed seedlings. In all samples, NO2-OA was detectable at nanomolar concentrations. The seeds showed the highest NO2-OA content, which decreased during germination. In contrast, nitric oxide (●NO) levels increased in the early stages of germination and seedling growth. Exogenous NO2-OA treatment (100 µM, 24 h) of Brassica seeds resulted in significantly increased ●NO level and induced germination capacity compared to untreated seeds. The results of in vitro approaches (4-Amino-5-methylamino-2’,7’-difluorofluorescein (DAF-FM) fluorescence, ●NO -sensitive electrode) supported the ●NO liberating capacity of NO2-OA. We observed for the first time that Brassica seeds and seedlings contain free NO2-OA which may be involved in germination as an ●NO donor as suggested both by the results of exogenous NO2-OA treatment of seeds and in vitro approaches. Due to their high NO2- OA content, Brassica sprouts can be considered as a good source of dietary NO2-OA intake. |
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| Terjedelem/Fizikai jellemzők: | Azonosító: 406-Terjedelem: 17 p |
| ISSN: | 2223-7747 |