Synthesis of N-Peptide-6-Amino-d-Luciferin Conjugates with Optimized Fragment Condensation Strategy
Abstract: The synthesis of peptide-luciferin conjugates has a pivotal role in the development of bioluminescent detection systems that are based on the determination of protease enzyme activity. This work describes the optimized synthesis of an N-peptide-6-amino-d-luciferin conjugate (Fmoc-Gly-Pro-6...
Elmentve itt :
| Szerzők: | |
|---|---|
| Dokumentumtípus: | Cikk |
| Megjelent: |
2019
|
| Sorozat: | INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
25 No. 3 |
| doi: | 10.1007/s10989-018-9768-8 |
| mtmt: | 30382079 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/16955 |
| LEADER | 01789nab a2200265 i 4500 | ||
|---|---|---|---|
| 001 | publ16955 | ||
| 005 | 20191007121404.0 | ||
| 008 | 191007s2019 hu o 0|| zxx d | ||
| 022 | |a 1573-3149 | ||
| 024 | 7 | |a 10.1007/s10989-018-9768-8 |2 doi | |
| 024 | 7 | |a 30382079 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a zxx | ||
| 100 | 1 | |a Kovács Anita Kármen | |
| 245 | 1 | 0 | |a Synthesis of N-Peptide-6-Amino-d-Luciferin Conjugates with Optimized Fragment Condensation Strategy |h [elektronikus dokumentum] / |c Kovács Anita Kármen |
| 260 | |c 2019 | ||
| 300 | |a 1209-1215 | ||
| 490 | 0 | |a INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS |v 25 No. 3 | |
| 520 | 3 | |a Abstract: The synthesis of peptide-luciferin conjugates has a pivotal role in the development of bioluminescent detection systems that are based on the determination of protease enzyme activity. This work describes the optimized synthesis of an N-peptide-6-amino-d-luciferin conjugate (Fmoc-Gly-Pro-6-amino-d-luciferin) with a simple fragment condensation method in adequate yields. Fmoc-Gly-Pro-6-amino-d-luciferin was produced from a previously synthesized Fmoc-Gly-Pro-OH and also previously synthesized 6-amino-2-cyanobenzothiazole with an optimized method, to which conjugate cysteine was added in an also improved way. The resulting conjugate was successfully used in a bioluminescent system, in vitro, demonstrating the applicability of the method. Graphical Abstract: [Figure not available: see fulltext.]. © 2018, Springer Nature B.V. | |
| 700 | 0 | 1 | |a Hegyes Péter |e aut |
| 700 | 0 | 1 | |a Szebeni Gábor |e aut |
| 700 | 0 | 1 | |a Bogár Krisztián |e aut |
| 700 | 0 | 1 | |a Puskás László |e aut |
| 700 | 0 | 1 | |a Tóth Gábor |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/16955/1/Kovacs2018_IJPRT.pdf |z Dokumentum-elérés |