Synthesis of N-Peptide-6-Amino-d-Luciferin Conjugates with Optimized Fragment Condensation Strategy
Abstract: The synthesis of peptide-luciferin conjugates has a pivotal role in the development of bioluminescent detection systems that are based on the determination of protease enzyme activity. This work describes the optimized synthesis of an N-peptide-6-amino-d-luciferin conjugate (Fmoc-Gly-Pro-6...
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| Dokumentumtípus: | Cikk |
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2019
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| Sorozat: | INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
25 No. 3 |
| doi: | 10.1007/s10989-018-9768-8 |
| mtmt: | 30382079 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/16955 |
| Tartalmi kivonat: | Abstract: The synthesis of peptide-luciferin conjugates has a pivotal role in the development of bioluminescent detection systems that are based on the determination of protease enzyme activity. This work describes the optimized synthesis of an N-peptide-6-amino-d-luciferin conjugate (Fmoc-Gly-Pro-6-amino-d-luciferin) with a simple fragment condensation method in adequate yields. Fmoc-Gly-Pro-6-amino-d-luciferin was produced from a previously synthesized Fmoc-Gly-Pro-OH and also previously synthesized 6-amino-2-cyanobenzothiazole with an optimized method, to which conjugate cysteine was added in an also improved way. The resulting conjugate was successfully used in a bioluminescent system, in vitro, demonstrating the applicability of the method. Graphical Abstract: [Figure not available: see fulltext.]. © 2018, Springer Nature B.V. |
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| Terjedelem/Fizikai jellemzők: | 1209-1215 |
| ISSN: | 1573-3149 |