Rutin and Its Cyclodextrin Inclusion Complexes Physico-chemical Evaluation and in vitro Activity on B164A5 Murine Melanoma Cell Line. /
BACKGROUND: Recent studies have shown that rutin presents a heightened interest due to the plethora of biological activities. The major drawback for this phytocompound is the poor water solubility, a parameter that limits its bioavailability. The study aimed to prepare and assess the inclusion compl...
Elmentve itt :
| Szerzők: | |
|---|---|
| Dokumentumtípus: | Cikk |
| Megjelent: |
2017
|
| Sorozat: | CURRENT PHARMACEUTICAL BIOTECHNOLOGY
18 No. 13 |
| doi: | 10.2174/1389201019666180209165523 |
| mtmt: | 3375794 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/14777 |
| Tartalmi kivonat: | BACKGROUND: Recent studies have shown that rutin presents a heightened interest due to the plethora of biological activities. The major drawback for this phytocompound is the poor water solubility, a parameter that limits its bioavailability. The study aimed to prepare and assess the inclusion complexes of rutin with beta-cyclodextrin (BCD) and hydroxypropyl-beta-cyclodextrin (HPBCD), followed by the evaluation of the antioxidant, antiproliferative and pro-apoptotic activity against B164A5 murine melanoma cell line. METHODS: Inclusion complexes were prepared by kneading method. Scanning electron microscopy, differential scanning calorimetry and X-ray powder diffraction were used in order to assess their formation. Antioxidant activity was determined by DPPH assay. Antiproliferative and pro-apoptotic effects where tested by MTT, respectively Annexin V-PI assays. Physico-chemical methods proved that incorporation took place. RESULTS: Results show that rutin presents antioxidant activity, and complexation increases this property. After 72 h of incubation at the concentration of 100 microM rutin proved to be an active antiproliferative and pro-apoptotic compound against B164A5 cells. CONCLUSION: Incorporation in BCD and HBCD maintained the activity, thus representing an important finding and open path for in vivo experiments. |
|---|---|
| Terjedelem/Fizikai jellemzők: | 1067-1077 |
| ISSN: | 1389-2010 |