The proteomic profile of a mouse model of proliferative vitreoretinopathy
Proliferative vitreoretinopathy (PVR) develops as a complication of retinal detachment surgery and represents a devastating condition leading to serious vision loss. A good animal model that permits extensive functional studies and drug testing is crucial in finding better therapeutic modalities for...
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Dokumentumtípus: | Cikk |
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Elsevier
2017
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Sorozat: | FEBS OPEN BIO
7 No. 8 |
doi: | 10.1002/2211-5463.12252 |
mtmt: | 3255497 |
Online Access: | http://publicatio.bibl.u-szeged.hu/12222 |
Tartalmi kivonat: | Proliferative vitreoretinopathy (PVR) develops as a complication of retinal detachment surgery and represents a devastating condition leading to serious vision loss. A good animal model that permits extensive functional studies and drug testing is crucial in finding better therapeutic modalities for PVR. A previously established mouse model, using dispase injection, was analyzed from the proteomic point of view, examining global protein profile changes by 2D electrophoresis, image analysis and HPLC-tandem mass spectrometry-based protein identification. The easy applicability of the mouse model was used to study the role of transglutaminase 2 (TG2) in PVR formation by proteomic examination of dispase-induced TG2 knockout vitreous samples. Our data demonstrate that, despite the altered appearance of crystallin proteins, the lack of TG2 did not prevent the development of PVR. |
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Terjedelem/Fizikai jellemzők: | 1166-1177 |
ISSN: | 2211-5463 |