Neocortical c-fos mRNA transcription in repeated, brief, acute seizures is c-fos a coincidence detector? /
The effect of acute brief seizures on neocortical c-fos expression was investigated in rats injected with 5 mg/kg 4- aminopyridine. Electroencephalography in freely moving animals with implanted neocortical electrodes detected an average of 2.67 tonic-clonic convulsions within 1 h following the 4...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2005
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| Sorozat: | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
15 No. 3 |
| mtmt: | 1300387 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/10887 |
| Tartalmi kivonat: | The effect of acute brief seizures on neocortical c-fos expression was investigated in rats injected with 5 mg/kg 4- aminopyridine. Electroencephalography in freely moving animals with implanted neocortical electrodes detected an average of 2.67 tonic-clonic convulsions within 1 h following the 4-AP treatment. Tissue samples of the somatosensory neocortex were collected at 30 min, 1 h, 3 h, 5 h and 8 h following the treatment for PCR and immunohistochemistry. The c-fos mRNA displayed the first significant rise at 1 h, and remained significantly higher through 3 h. The number of c-fos protein immunoreactive cells was significantly elevated already at 30 min, peaked at 1 h, and declined by 5 h. We conclude that in repetitive, brief seizures, the first convulsion does not increase c-fos RNA transcription, whilst the second causes a long-lasting gene expression and a large increase of c-fos protein synthesis. The phenomenon may have implications in the pathogenesis of human and animal epilepsies. |
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| Terjedelem/Fizikai jellemzők: | 481-486 |
| ISSN: | 1107-3756 |