The neonatal sarcoplasmic reticulum Ca-ATPase gives a clue to development and pathology in human muscles
The sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (SERCA1) has two muscle specific splice isoforms; SERCA1a in fast-type adult and SERCA1b in neonatal and regenerating skeletal muscles. At the protein level the only difference between these two isoforms is that SERCA1a has C-terminal glycine...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
Chapman and Hall
2015
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| Sorozat: | JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY
36 No. 2 |
| doi: | 10.1007/s10974-014-9403-z |
| mtmt: | 2790554 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/10871 |
| Tartalmi kivonat: | The sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (SERCA1) has two muscle specific splice isoforms; SERCA1a in fast-type adult and SERCA1b in neonatal and regenerating skeletal muscles. At the protein level the only difference between these two isoforms is that SERCA1a has C-terminal glycine while SERCA1b has an octapeptide tail instead. This makes the generation of a SERCA1a specific antibody not feasible. The switch between the two isoforms is a hallmark of differentiation so we describe here a method based on the signal ratios of the SERCA1b specific and pan SERCA1 antibodies to estimate the SERCA1b/SERCA1a dominance on immunoblot of human muscles. Using this method we showed that unlike in mouse and rat, SERCA1b was only expressed in pre-matured infant leg and arm muscles; it was replaced by SERCA1a in more matured neonatal muscles and was completely absent in human foetal and neonatal diaphragms. Interestingly, only SERCA1a and no SERCA1b were detected in muscles of 7-12 years old boys with Duchenne, a degenerative-regenerative muscular dystrophy. However, in adult patients with myotonic dystrophy type 2 (DM2), the SERCA1b dominated over SERCA1a. Thus the human SERCA1b has a different expression pattern from that of rodents and it is associated with DM2. |
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| Terjedelem/Fizikai jellemzők: | 195-203 |
| ISSN: | 0142-4319 |