Neuronal tumour necrosis factor-alpha and interleukin-1beta expression in a porcine model of intracerebral haemorrhage Modulation by U-74389G /
Tumour necrosis factor alpha (TNF-alpha) and interleukin 1beta (IL-1beta) are important mediators of intracerebral haemorrhage (ICH) inflammatory response. Lazaroids, established antioxidants and neuroprotectants, have been studied in several brain pathologies. The present study was designed to inve...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
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Elsevier
2015
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| Sorozat: | BRAIN RESEARCH
1615 |
| doi: | 10.1016/j.brainres.2015.04.034 |
| mtmt: | 2907577 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/10159 |
| Tartalmi kivonat: | Tumour necrosis factor alpha (TNF-alpha) and interleukin 1beta (IL-1beta) are important mediators of intracerebral haemorrhage (ICH) inflammatory response. Lazaroids, established antioxidants and neuroprotectants, have been studied in several brain pathologies. The present study was designed to investigate: a) TNF-alpha and IL-1beta changes, in neurons and b) U-74389G effects, 4 and 24h after haematoma induction in a porcine model of intracerebral haemorrhage. In twenty male landrace pigs (swines) aged 135-150 days old, autologous whole blood was injected around the right basal ganglia territory; in ten of the pigs the lazaroid compound U-74389G was administered. Brain TNF-alpha and IL-1beta immunopositive neurons were determined by immunoarray techniques at 4 and 24h timepoints. After the haematoma induction the number of TNF-alpha immunopositive neurons ipsilateral to the haematoma was significantly higher compared to the contralateral site at 4h (p<0.0005), while U-74389G significantly reduced the number of TNF-alpha immunopositive neurons, ipsilateral to the haematoma, at 4h (p=0.002); at 24h, TNF-alpha immunopositive neurons were found significantly lower in the control group ipsilateral to the haematoma in comparison to 4h timepoint(p<0.0005). The number of IL-1beta immunopositive neurons at 4h after the hematoma induction was significantly higher ipsilateral to the haematoma site (p<0.0005). U-74389G had no statistical significant effect. TNF-alpha and IL-1beta, increase in neurons, 4h after the haematoma induction, ipsilateral to the haematoma site. The administration of the antioxidant compound U-74389G, results in early (at 4h) decrease of TNF-alpha immunopositive neurons but shows no statistical significant effect to IL-1beta immunopossitive neurons. |
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| Terjedelem/Fizikai jellemzők: | 98-105 |
| ISSN: | 0006-8993 |