Functional diversity investigation of bacterial communities in distinct soil types with RISA after preculturing (RISA-APC) method
Microorganisms play a leading role in soil development and preservation; moreover, they could indicate the soil health and conditions. In this study, we analyzed the bacterial composition of three different soil types with a newly developed RISA-APC method. A novelty of this method is the pre-cultur...
Elmentve itt :
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| Testületi szerző: | |
| Dokumentumtípus: | Cikk |
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Szegedi Tudományegyetem Mezőgazdasági Kar
Szeged
2011
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| Sorozat: | Agrár- és vidékfejlesztési szemle
6 No. 1 |
| Kulcsszavak: | Mikrobiológia, Talaj |
| Tárgyszavak: | |
| Online Access: | http://acta.bibl.u-szeged.hu/76132 |
| Tartalmi kivonat: | Microorganisms play a leading role in soil development and preservation; moreover, they could indicate the soil health and conditions. In this study, we analyzed the bacterial composition of three different soil types with a newly developed RISA-APC method. A novelty of this method is the pre-culturing step: this preculturing of the bacterial communities were performed on solid media supplemented with different carbon sources (e.g.: carboxy-methyl cellulose, xylan, chitin, starch, tributyrin, casein and protocatechuic acid). For the investigation of heavy metal tolerant bacteria, a preculturing on YEG media containing CuSO4 or CdCl2 were used. The mini-colonies developed after a short incubation time was investigated. This RISA-APC method proved to be a useful tool for the comparison of different soil types, and for the examination of changes in the soil bacterial community structure. It was clearly shown that the most diverse functional diversity values occurred in the forest soil and the less diverse bacterial community was detected in sandy soil samples. |
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| Terjedelem/Fizikai jellemzők: | 351-355 |
| ISSN: | 1788-5345 |