Chlorophyll fluorescence instrumentation for a rapid, in situ measurement of algal density

In the project reported, we are developing an instrument for measuring algal density based on the detection of chlorophyll fluorescence. Following the adjustment of several parameters defined during preliminary analyses, measurements were made on different concentrations of model green and blue alga...

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Bibliographic Details
Main Authors: Lázár Diána
Klátyik Szandra
Lenk Sándor
Barócsi Attila
Kocsányi László
Adányi Nóra
Takács Eszter
Székács András
Corporate Author: International Symposium on Analytical and Environmental Problems (26.) (2020) (Szeged)
Format: Book part
Published: 2020
Series:Proceedings of the International Symposium on Analytical and Environmental Problems 26
Kulcsszavak:Kémia
Subjects:
Online Access:http://acta.bibl.u-szeged.hu/73998
Description
Summary:In the project reported, we are developing an instrument for measuring algal density based on the detection of chlorophyll fluorescence. Following the adjustment of several parameters defined during preliminary analyses, measurements were made on different concentrations of model green and blue algal cultures. Fluorescent signal intensities measured by the prototypes of the fluorometer module were compared to values determined by other, widely used methods for estimation of algal density (i.e. Bürker chamber cell counting, optical density measurement and chlorophyll-a measurement with ethanol extraction method). Fluorometer results correlated well with the other methods, resulting high correlation coefficients (R2>0.9%). Limits of detection and limits of quantification showed a decreasing trend during the development phases resulting in a highly sensitive instrument.
Physical Description:211-215
ISBN:978-963-306-771-0