Concomitant yield optimization of tannase and gallic acid by Bacillus licheniformis KBR6 through submerged fermentation an industrial approach /
The present study is concerned with the evaluation of tannase and gallic acid production effi cacy of Bacillus licheniformis KBR6 under diff erent environmental conditions through submerged fermentation. Results have shown that diff erent environmental conditions and mineral sources have diff erenti...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2020
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| Sorozat: | Acta biologica Szegediensis
64 No. 2 |
| Kulcsszavak: | Mikrobiológia |
| Tárgyszavak: | |
| doi: | 10.14232/abs.2020.2.151-158 |
| Online Access: | http://acta.bibl.u-szeged.hu/73065 |
| Tartalmi kivonat: | The present study is concerned with the evaluation of tannase and gallic acid production effi cacy of Bacillus licheniformis KBR6 under diff erent environmental conditions through submerged fermentation. Results have shown that diff erent environmental conditions and mineral sources have diff erential infl uences on tannase and gallic acid production. Highest tannase and gallic acid yield was observed at incubation period of 18 h and 22 h, respectively. At tannic acid concentration of 15 g/l, maximum cell mass (0.75 g/l), cell yield coeffi cient (0.08 g/g), specifi c growth rate (37.5 mg/g/h), tannase yield (16.3 U/g) and specifi c tannase production rate (0.80 U/g/h) were observed, however, at higher tannic acid concentration a decrease in tannase yield and production rate were observed, but gallic acid production increased with increasing tannic acid concentration. Additional carbohydrate sources like glucose, fructose, and lactose showed positive infl uence on enzyme yield. Among the studied nitrogen sources urea and NH4Cl, and of the phosphate sources KH2PO4 showed favourable eff ects on cell growth and simultaneous enzyme and gallic acid production. Temperature of 35 °C was found to be optimum for tannase and gallic acid production. Of all the studied metal ions Ca2+, Mg2+ and Na+ showed positive eff ect whereas, Co2+, Ag2+, Pb2+, Hg2+ showed inhibitory eff ects. |
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| Terjedelem/Fizikai jellemzők: | 151-158 |
| ISSN: | 1588-4082 |