Expression and production optimization of the cationic antimicrobial peptide indolicidin by the recombinant E. coli C41 (DE3) clones /

The cytoplasmic granules of bovine neutrophils naturally possess indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask level production and expression optimizations of the indol...

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Bibliographic Details
Main Authors: Selvam Kanesan Panneer
Nambirajan Guruvu
Annamalai Balasubramaniam
Alaidarous Mohammed
Alshehri Bader Mohammed
Bin Dukhyil Abdul Aziz A.
Shobana Coimbatore Subramanian
Manikandan Palanisamy
Format: Article
Published: University of Szeged Szeged 2018
Series:Acta biologica Szegediensis 62 No. 1
Kulcsszavak:Mikrobiológia
doi:10.14232/abs.2018.1.61-66

Online Access:http://acta.bibl.u-szeged.hu/58199
Description
Summary:The cytoplasmic granules of bovine neutrophils naturally possess indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask level production and expression optimizations of the indolicidin by the recombinant Escherichia coli C41 (DE3) clones (transformed with pET21a(+) plasmid carrying indolicidin gene) were carried out under standard conditions, as to determine the conditions required for maximal production. It was determined that a concentration of 1 mM of IPTG was effective, the 2×YT with salts and LB media at pH 7.5 with 3-6 h of incubation were required for maximal indolicidin expression.
Physical Description:61-66
ISSN:1588-4082