Application of laccases, produced by ganoderma species, for the detoxification of some aniline and phenol derivatives
The objective of this work was to collect isolates of the white rot fungus Ganoderma from decaying woods and investigate their laccase producing and detoxification abilities against aniline and phenol compounds. Sporocarps were collected and the tissue culturing technique was used for fungal isolati...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2013
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| Sorozat: | Review on agriculture and rural development
2 No. 1 |
| Kulcsszavak: | Mikrobiológia, Analitikai kémia - enzimek, Bioinformatika |
| Tárgyszavak: | |
| Online Access: | http://acta.bibl.u-szeged.hu/55293 |
| Tartalmi kivonat: | The objective of this work was to collect isolates of the white rot fungus Ganoderma from decaying woods and investigate their laccase producing and detoxification abilities against aniline and phenol compounds. Sporocarps were collected and the tissue culturing technique was used for fungal isolation. In these experiments specific medium, containing benomyl, dichloran and guaiacol was used. Laccase producing of the isolates were tested in liquid media containing different inducers. Crude enzyme extracts were prepared and characterized. The best laccase-producing strains were identified with partial ITS sequence analysis. Their secreted laccases were investigated with SDS-PAGE and the molecular weights of these enzymes were estimated. Degradation of 7 different aniline and phenol derivates (2,4-dichlorophenol, 2-methyl-4- chlorophenol, 3-chloroaniline, 4-chloroaniline, 2,6-dimethylaniline, 3,4-dichloroaniline and 3-chloro-4- methylaniline) were investigated, observing high degradation rates in most cases. |
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| Terjedelem/Fizikai jellemzők: | 422-426 |
| ISSN: | 2063-4803 |