Attempts to produce transgenic Beta vulgaris L. plants via combined gene transfer methods
We have started to elaborate a general transformation and plant regeneration system for Beta vulgaris L. using combined gene transfer procedure. Three methods were tested: particle bombardment with pure wolfram micro-carriers prior to Agrobacterium treatment, vortexing and infiltration of explants i...
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| Dokumentumtípus: | Cikk |
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2002
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| Sorozat: | Acta biologica Szegediensis
46 No. 3-4 |
| Kulcsszavak: | Természettudomány, Biológia |
| Online Access: | http://acta.bibl.u-szeged.hu/22493 |
| Tartalmi kivonat: | We have started to elaborate a general transformation and plant regeneration system for Beta vulgaris L. using combined gene transfer procedure. Three methods were tested: particle bombardment with pure wolfram micro-carriers prior to Agrobacterium treatment, vortexing and infiltration of explants in Agrobacterium suspension, halving of explants. The histochemical analysis of transient and stabile gus-gene expression showed the beneficial effect of explant halving and infiltration in Agrobacterium suspension. |
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| Terjedelem/Fizikai jellemzők: | 43-44 |
| ISSN: | 1588-385X |