Non-thermal effect of microwave treatment on enzyme suspensions part II. : cellulase enzyme activity /
Renewable fuel production (the second generation bio-ethanol production) is one of the key areas of the EU 7th Framework Programme . One of the challenges in this research area is that the degradation of cellulose materials into glucose is very expensive at the moment. Our research aim was to enhanc...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
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2009
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| Sorozat: | Review of faculty of engineering : analecta technica Szegedinensia
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| Kulcsszavak: | Mérnöki tudományok |
| Online Access: | http://acta.bibl.u-szeged.hu/11796 |
| Tartalmi kivonat: | Renewable fuel production (the second generation bio-ethanol production) is one of the key areas of the EU 7th Framework Programme . One of the challenges in this research area is that the degradation of cellulose materials into glucose is very expensive at the moment. Our research aim was to enhance the activity of cellobiohydrolase enzyme using only physical methods. At the beginning of this research we used D-(+)-cellobiose as substrate. During treatments a special designed inverter type microwave oven wa s used running on 50 W. In this equipment the temperature of enzyme-substrate solution was increased until 45 °C. As control measurement the suspension was heated up on a hot-plate until 45 °C. After the treatment samples were incubated at 37 °C. Enzyme activity was measured at 505 nm by determining glucose concentration. Based on the results the produced glucose of the microwave treated solution was 20 % higher than in the solution heated up on a hot-plate. |
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| Terjedelem/Fizikai jellemzők: | 63-68 |
| ISSN: | 1788-6392 |