Extreme specificity of NCR gene expression in Medicago truncatula

Background: Legumes form root nodules to house nitrogen fixing bacteria of the rhizobium family. The rhizobia are located intracellularly in the symbiotic nodule cells. In the legume Medicago truncatula these cells produce high amounts of Nodule-specific Cysteine-Rich (NCR) peptides which induce dif...

Teljes leírás

Elmentve itt :
Bibliográfiai részletek
Szerzők: Guefrachi Ibtissem
Nagymihály Marianna
Pislariu Catalina I.
Van de Velde Willem
Ratet Pascal
Mars Mohamed
Udvardi Michael K.
Kondorosi Éva
Mergaert Peter
Alunni Benoît
Dokumentumtípus: Cikk
Megjelent: Biomed Central Ltd. 2014
Sorozat:BMC GENOMICS 15
doi:10.1186/1471-2164-15-712

mtmt:2816012
Online Access:http://publicatio.bibl.u-szeged.hu/9229
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245 1 0 |a Extreme specificity of NCR gene expression in Medicago truncatula  |h [elektronikus dokumentum] /  |c  Guefrachi Ibtissem 
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490 0 |a BMC GENOMICS  |v 15 
520 3 |a Background: Legumes form root nodules to house nitrogen fixing bacteria of the rhizobium family. The rhizobia are located intracellularly in the symbiotic nodule cells. In the legume Medicago truncatula these cells produce high amounts of Nodule-specific Cysteine-Rich (NCR) peptides which induce differentiation of the rhizobia into enlarged, polyploid and non-cultivable bacterial cells. NCRs are similar to innate immunity antimicrobial peptides. The NCR gene family is extremely large in Medicago with about 600 genes. Results: Here we used the Medicago truncatula Gene Expression Atlas (MtGEA) and other published microarray data to analyze the expression of 334 NCR genes in 267 different experimental conditions. We find that all but five of these genes are expressed in nodules but in no other plant organ or in response to any other biotic interaction or abiotic stress tested. During symbiosis, none of the genes are induced by Nod factors. The NCR genes are activated in successive waves during nodule organogenesis, correlated with bacterial infection of the nodule cells and with a specific spatial localization of their transcripts from the apical to the proximal nodule zones. However, NCR expression is not associated with nodule senescence. According to their Shannon entropy, a measure expressing tissue specificity of gene expression, the NCR genes are among the most specifically expressed genes in M. truncatula. Moreover, when activated in nodules, their expression level is among the highest of all genes. Conclusions: Together, these data show that the NCR gene expression is subject to an extreme tight regulation and is only activated during nodule organogenesis in the polyploid symbiotic cells. 
700 0 1 |a Nagymihály Marianna  |e aut 
700 0 1 |a Pislariu Catalina I.  |e aut 
700 0 2 |a Van de Velde Willem  |e aut 
700 0 2 |a Ratet Pascal  |e aut 
700 0 2 |a Mars Mohamed  |e aut 
700 0 2 |a Udvardi Michael K.  |e aut 
700 0 2 |a Kondorosi Éva  |e aut 
700 0 2 |a Mergaert Peter  |e aut 
700 0 2 |a Alunni Benoît  |e aut 
856 4 0 |u http://publicatio.bibl.u-szeged.hu/9229/1/Guefrachi2014.pdf  |z Dokumentum-elérés