Hippocampal Sclerosis in Pilocarpine Epilepsy Survival of Peptide-Containing Neurons and Learning and Memory Disturbances in the Adult NMRI Strain Mouse /
The present experiments reveal the alterations of the hippocampal neuronal populations in chronic epilepsy. The mice were injected with a single dose of pilocarpine. They had status epilepticus and spontaneously recurrent motor seizures. Three months after pilocarpine treatment, the animals were inv...
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2022
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| Sorozat: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
23 No. 1 |
| Tárgyszavak: | |
| doi: | 10.3390/ijms23010204 |
| mtmt: | 32641219 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/39239 |
| LEADER | 02472nab a2200241 i 4500 | ||
|---|---|---|---|
| 001 | publ39239 | ||
| 005 | 20260221154048.0 | ||
| 008 | 260221s2022 hu o 000 eng d | ||
| 022 | |a 1661-6596 | ||
| 024 | 7 | |a 10.3390/ijms23010204 |2 doi | |
| 024 | 7 | |a 32641219 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a eng | ||
| 100 | 1 | |a Mátyás Adrienne | |
| 245 | 1 | 0 | |a Hippocampal Sclerosis in Pilocarpine Epilepsy |h [elektronikus dokumentum] : |b Survival of Peptide-Containing Neurons and Learning and Memory Disturbances in the Adult NMRI Strain Mouse / |c Mátyás Adrienne |
| 260 | |c 2022 | ||
| 300 | |a 19 | ||
| 490 | 0 | |a INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |v 23 No. 1 | |
| 520 | 3 | |a The present experiments reveal the alterations of the hippocampal neuronal populations in chronic epilepsy. The mice were injected with a single dose of pilocarpine. They had status epilepticus and spontaneously recurrent motor seizures. Three months after pilocarpine treatment, the animals were investigated with the Barnes maze to determine their learning and memory capabilities. Their hippocampi were analyzed 2 weeks later (at 3.5 months) with standard immunohistochemical methods and cell counting. Every animal displayed hippocampal sclerosis. The neuronal loss was evaluated with neuronal‐N immunostaining, and the activation of the microglia was measured with Iba1 immunohistochemistry. The neuropeptide Y, parvalbumin, and calretinin immunoreactive structures were qualitatively and quantitatively analyzed in the hippocampal formation. The results were compared statistically to the results of the control mice. We detected neuronal loss and strongly activated microglia populations. Neuropeptide Y was significantly upregulated in the sprouting axons. The number of parvalbumin‐ and calretinin‐containing interneurons decreased significantly in the Ammon’s horn and dentate gyrus. The epileptic animals displayed significantly worse learning and memory functions. We concluded that degeneration of the principal neurons, a numerical decrease of PV‐containing GABAergic neurons, and strong peptidergic axonal sprouting were responsible for the loss of the hippocampal learning and memory functions. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | |
| 650 | 4 | |a Általános orvostudomány | |
| 700 | 0 | 1 | |a Borbély Emőke |e aut |
| 700 | 0 | 1 | |a Mihály András |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/39239/1/32641219.pdf |z Dokumentum-elérés |