First synthesis of human gastrin-II and radiolabeled cholecystokinin-octapeptides
Human gastrin I pyroGlu-Gly-Pro-Trp-Leu-(Glu)5-Ala-Tyr(R)-Gly-Trp-Met-Asp-Phe-NH2 (I; R = H) (II) was prepd. by the solid-phase method on an α-methylbenzylhydrylamine resin and then it was o-sulfated with pyridine-SO3 or pyridinium acetylsulfate (PAS) in pyridine-DMF to give human gastrin II (I, R =...
Elmentve itt :
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| Testületi szerző: | |
| Dokumentumtípus: | Könyv része |
| Megjelent: |
Almqvist & Wiksell International
Stockholm
1984
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| Sorozat: | Peptides 1984
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| Tárgyszavak: | |
| mtmt: | 1876160 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/33243 |
| LEADER | 02294naa a2200325 i 4500 | ||
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| 005 | 20250524152330.0 | ||
| 008 | 250524s1984 hu o 100 eng d | ||
| 024 | 7 | |a 1876160 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zarándi Márta | |
| 245 | 1 | 0 | |a First synthesis of human gastrin-II and radiolabeled cholecystokinin-octapeptides |h [elektronikus dokumentum] / |c Zarándi Márta |
| 260 | |a Almqvist & Wiksell International |b Stockholm |c 1984 | ||
| 300 | |a 4 | ||
| 300 | |a 387-390 | ||
| 490 | 0 | |a Peptides 1984 | |
| 520 | 3 | |a Human gastrin I pyroGlu-Gly-Pro-Trp-Leu-(Glu)5-Ala-Tyr(R)-Gly-Trp-Met-Asp-Phe-NH2 (I; R = H) (II) was prepd. by the solid-phase method on an α-methylbenzylhydrylamine resin and then it was o-sulfated with pyridine-SO3 or pyridinium acetylsulfate (PAS) in pyridine-DMF to give human gastrin II (I, R = SO3H) (III). II and III were also prepd. by classical soln. methods using minimal side-chain protection; the presence of 5 free γ-carboxyl groups in sequence 6-11 caused problems in azide formation and decreased the yield. The solid-phase synthesis of cholecystokinin octapeptide (CCK-8), H-Asp-X-Met-Gly-Trp-Met-Asp-Phe-NH2 [IV, X = Tyr(SO3H)], and caerulein was optimized using Trp(CHO) and Met(O) for protection and PAS for O-sulfation. Tritiated CCK-8 desulfate [IV, X = Tyr(3,5-3H)] was prepd. from IV [X = Tyr(3,5-I2)] (V) by an exchange reaction with 3H2/PdO; V was prepd. by the solid-phase method on a benzhydrylamine resin. Due to steric hindrance, the synthesis of tritiated CCK-8 failed; therefore, Nα-Fmoc-CCK-8 desulfate (Fmoc = 9-fluorenylmethoxycarbonyl) was prepd. by the solid-phase method and then it was O-sulfated with 35S-PAS and Fmoc-deblocked by Tesser's base to give 35S-labeled CCK-8. [on SciFinder(R)] | |
| 650 | 4 | |a Kémiai tudományok | |
| 650 | 4 | |a Általános orvostudomány | |
| 700 | 0 | 1 | |a Penke Botond |e aut |
| 700 | 0 | 1 | |a Zsigo J. |e aut |
| 700 | 0 | 1 | |a Varga J. |e aut |
| 700 | 0 | 1 | |a Toth G. |e aut |
| 700 | 0 | 1 | |a Pelczer I. |e aut |
| 700 | 0 | 1 | |a Rivier J. |e aut |
| 700 | 0 | 1 | |a Kovacs K. |e aut |
| 700 | 0 | 1 | |a Tóth Gábor |e aut |
| 711 | |a Peptides 1984 |c Djurönäset |d 1984.06.10-1984.06.15 | ||
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/33243/1/aok_klny_1352_84.pdf |z Dokumentum-elérés |