Galectin-1 as a marker for microglia activation in the aging brain
Microglia cells, the immune cells residing in the brain, express immune regulatory molecules that have a central role in the manifestation of age-related brain characteristics. Our hypothesis suggests that galectin-1, an anti-inflammatory member of the beta-galactoside-binding lectin family, regulat...
Elmentve itt :
| Szerzők: | |
|---|---|
| Dokumentumtípus: | Cikk |
| Megjelent: |
2023
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| Sorozat: | BRAIN RESEARCH
1818 |
| Tárgyszavak: | |
| doi: | 10.1016/j.brainres.2023.148517 |
| mtmt: | 34093747 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/29745 |
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| 100 | 1 | |a Kiss Tamás | |
| 245 | 1 | 0 | |a Galectin-1 as a marker for microglia activation in the aging brain |h [elektronikus dokumentum] / |c Kiss Tamás |
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| 520 | 3 | |a Microglia cells, the immune cells residing in the brain, express immune regulatory molecules that have a central role in the manifestation of age-related brain characteristics. Our hypothesis suggests that galectin-1, an anti-inflammatory member of the beta-galactoside-binding lectin family, regulates microglia and neuroinflammation in the aging brain. Through our in-silico analysis, we discovered a subcluster of microglia in the aged mouse brain that exhibited increased expression of galectin-1 mRNA. In our Western blotting experiments, we observed a decrease in galectin-1 protein content in our rat primary cortical cultures over time. Additionally, we found that the presence of lipopolysaccharide, an immune activator, significantly increased the expression of galectin-1 protein in microglial cells. Utilizing flow cytometry, we determined that a portion of the galectin-1 protein was localized on the surface of the microglial cells. As cultivation time increased, we observed a decrease in the expression of activation-coupled molecules in microglial cells, indicating cellular exhaustion. In our mixed rat primary cortical cell cultures, we noted a transition of amoeboid microglial cells labeled with OX42(CD11b/c) to a ramified, branched phenotype during extended cultivation, accompanied by a complete disappearance of galectin-1 expression. By analyzing the transcriptome of a distinct microglial subpopulation in an animal model of aging, we established a correlation between chronological aging and galectin-1 expression. Furthermore, our in vitro study demonstrated that galectin-1 expression is associated with the functional activation state of microglial cells exhibiting specific amoeboid morphological characteristics. Based on our findings, we identify galectin-1 as a marker for microglia activation in the context of aging. | |
| 650 | 4 | |a Klinikai orvostan | |
| 700 | 0 | 1 | |a Mir Mohd Yaqub |e aut |
| 700 | 0 | 1 | |a Stefancsik Gergely |e aut |
| 700 | 0 | 1 | |a Ganbat Gantulga |e aut |
| 700 | 0 | 1 | |a Askarova Aruzhan |e aut |
| 700 | 0 | 1 | |a Monostori Éva |e aut |
| 700 | 0 | 1 | |a Dulka Karolina |e aut |
| 700 | 0 | 1 | |a Szebeni Gábor |e aut |
| 700 | 0 | 2 | |a Nyúl-Tóth Ádám |e aut |
| 700 | 0 | 2 | |a Csiszar Anna |e aut |
| 700 | 0 | 2 | |a Légrádi Ádám |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/29745/1/1-s2.0-S0006899323002883-main.pdf |z Dokumentum-elérés |