03008nab a2200325 i 4500 publ27681 20230901113818.0 230629s2023 hu o 0|| Angol d 1474-9718 10.1111/acel.13894 doi 34035852 mtmt SZTE Publicatio Repozitórium hun Angol Barcena Maria Luisa Sex and age differences in AMPK phosphorylation, mitochondrial homeostasis, and inflammation in hearts from inflammatory cardiomyopathy patients [elektronikus dokumentum] / Barcena Maria Luisa 2023 15 AGING CELL 22 No. 8 Linked to exacerbated inflammation, myocarditis is a cardiovascular disease, which may lead to dilated cardiomyopathy. Although sex and age differences in the development of chronic myocarditis have been postulated, underlying cellular mechanisms remain poorly understood. In the current study, we aimed to investigate sex and age differences in mitochondrial homeostasis, inflammation, and cellular senescence. Cardiac tissue samples from younger and older patients with inflammatory dilated cardiomyopathy (DCMI) were used. The expression of Sirt1, phosphorylated AMPK, PGC-1α, Sirt3, acetylated SOD2, catalase, and several mitochondrial genes was analyzed to assess mitochondrial homeostasis. The expression of NF-κB, TLR4, and interleukins was used to examine the inflammatory state in the heart. Finally, several senescence markers and telomere length were investigated. Cardiac AMPK expression and phosphorylation were significantly elevated in male DCMI patients, whereas Sirt1 expression remained unchanged in all groups investigated. AMPK upregulation was accompanied by a preserved expression of all mitochondrial proteins/genes investigated in older male DCMI patients, whereas the expression of TOM40, TIM23, and the mitochondrial oxidative phosphorylation genes was significantly reduced in older female patients. Mitochondrial homeostasis in older male patients was further supported by the reduced acetylation of mitochondrial proteins as indicated by acetylated SOD2. The inflammatory markers NF-κB and TLR4 were downregulated in older male DCMI patients, whereas the expression of IL-18 was increased in older female patients. This was accompanied by progressed senescence in older DCMI hearts. In conclusion, older women experience more dramatic immunometabolic disorders on the cellular level than older men. Klinikai orvostan Tonini Greta aut Haritonow Natalie aut Breiter Pavelas aut Milting Hendrik aut Baczkó István aut Müller-Werdan Ursula aut Ladilov Yury aut Regitz-Zagrosek Vera aut http://publicatio.bibl.u-szeged.hu/27681/3/34035852_megjelent.pdf Dokumentum-elérés http://publicatio.bibl.u-szeged.hu/27681/1/Barcena.pdf Dokumentum-elérés