A series of xanthenes inhibiting Rad6 function and Rad6–Rad18 interaction in the PCNA ubiquitination cascade

Ubiquitination of proliferating cell nuclear antigen (PCNA) triggers pathways of DNA damage tolerance, including mutagenic translesion DNA synthesis, and comprises a cascade of reactions involving the E1 ubiquitin-activating enzyme Uba1, the E2 ubiquitin-conjugating enzyme Rad6, and the E3 ubiquitin...

Teljes leírás

Elmentve itt :
Bibliográfiai részletek
Szerzők: Fenteany Gabriel
Sharma Gaurav
Gaur Paras
Borics Attila
Wéber Edit
Kiss Ernő
Haracska Lajos
Dokumentumtípus: Cikk
Megjelent: 2022
Sorozat:ISCIENCE 25 No. 4
Tárgyszavak:
doi:10.1016/j.isci.2022.104053

mtmt:32746161
Online Access:http://publicatio.bibl.u-szeged.hu/26535
Leíró adatok
Tartalmi kivonat:Ubiquitination of proliferating cell nuclear antigen (PCNA) triggers pathways of DNA damage tolerance, including mutagenic translesion DNA synthesis, and comprises a cascade of reactions involving the E1 ubiquitin-activating enzyme Uba1, the E2 ubiquitin-conjugating enzyme Rad6, and the E3 ubiquitin ligase Rad18. We report here the discovery of a series of xanthenes that inhibit PCNA ubiquitination, Rad6-ubiquitin thioester formation, and the Rad6-Rad18 interaction. Structure-activity relationship experiments across multiple assays reveal chemical and structural features important for different activities along the pathway to PCNA ubiquitination. The compounds that inhibit these processes are all a subset of the xanthen-3-ones we tested. These small molecules thus represent first-in-class probes of Rad6 function and the association of Rad6 and Rad18, the latter being a new inhibitory activity discovered for a small molecule, in the PCNA ubiquitination cascade and potential therapeutic agents to contain cancer progression.
Terjedelem/Fizikai jellemzők:23
ISSN:2589-0042