Fine tuning of the catalytic activity of colicin E7 nuclease domain by systematic N-terminal mutations
The nuclease domain of colicin E7 (NColE7) promotes the nonspecific cleavage of nucleic acids at its C-terminal HNH motif. Interestingly, the deletion of four N-terminal residues (446-449 NColE7=KRNK) resulted in complete loss of the enzyme activity. R447A mutation was reported to decrease the nucle...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2014
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| Sorozat: | PROTEIN SCIENCE
23 No. 8 |
| doi: | 10.1002/pro.2497 |
| mtmt: | 2727141 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/18464 |