Tritium labelling of a cholesterol amphiphile designed for cell membrane anchoring of proteins.
Cell membrane association of proteins can be achieved by the addition of lipid moieties to the polypeptide chain, and such lipid-modified proteins have important biological functions. A class of cell surface proteins contains a complex glycosylphosphatidylinositol (GPI) glycolipid at the C-terminus,...
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2015
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| Sorozat: | JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
58 No. 1 |
| doi: | 10.1002/jlcr.3254 |
| mtmt: | 2824242 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/16996 |
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| 024 | 7 | |a 2824242 |2 mtmt | |
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| 100 | 1 | |a Schäfer Balázs | |
| 245 | 1 | 0 | |a Tritium labelling of a cholesterol amphiphile designed for cell membrane anchoring of proteins. |h [elektronikus dokumentum] / |c Schäfer Balázs |
| 260 | |c 2015 | ||
| 300 | |a 7-13 | ||
| 490 | 0 | |a JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS |v 58 No. 1 | |
| 520 | 3 | |a Cell membrane association of proteins can be achieved by the addition of lipid moieties to the polypeptide chain, and such lipid-modified proteins have important biological functions. A class of cell surface proteins contains a complex glycosylphosphatidylinositol (GPI) glycolipid at the C-terminus, and they are accumulated in cholesterol-rich membrane microdomains, that is, lipid rafts. Semisynthetic lipoproteins prepared from recombinant proteins and designed lipids are valuable probes and model systems of the membrane-associated proteins. Because GPI-anchored proteins can be reinserted into the cell membrane with the retention of the biological function, they are appropriate candidates for preparing models via reduction of the structural complexity. A synthetic headgroup was added to the 3beta-hydroxyl group of cholesterol, an essential lipid component of rafts, and the resulting cholesterol derivative was used as a simplified GPI mimetic. In order to quantitate the membrane integrated GPI mimetic after the exogenous addition to live cells, a tritium labelled cholesterol anchor was prepared. The radioactive label was introduced into the headgroup, and the radiolabelled GPI mimetic anchor was obtained with a specific activity of 1.37 TBq/mmol. The headgroup labelled cholesterol derivative was applied to demonstrate the sensitive detection of the cell membrane association of the anchor under in vivo conditions. | |
| 700 | 0 | 1 | |a Orbán Erika |e aut |
| 700 | 0 | 1 | |a Kele Zoltán |e aut |
| 700 | 0 | 1 | |a Tömböly Csaba |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/16996/1/Sch-fer_et_al-2015-Journal_of_Labelled_Compounds_and_Radiopharmaceuticals.pdf |z Dokumentum-elérés |