mmSTORM Multimodal localization based super-resolution microscopy /
Super-resolution localization microscopy provides a powerful tool to study biochemical mechanisms at single molecule level. Although the lateral position of the fluorescent dye molecules can be determined routinely with high precision, measurement of other modalities such as 3D and multicolor withou...
Elmentve itt :
| Szerzők: | |
|---|---|
| Dokumentumtípus: | Cikk |
| Megjelent: |
2019
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| Sorozat: | SCIENTIFIC REPORTS
9 |
| doi: | 10.1038/s41598-018-37341-9 |
| mtmt: | 30432741 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/15994 |
| LEADER | 01908nab a2200289 i 4500 | ||
|---|---|---|---|
| 001 | publ15994 | ||
| 005 | 20190704133546.0 | ||
| 008 | 190704s2019 hu o 0|| angol d | ||
| 022 | |a 2045-2322 | ||
| 024 | 7 | |a 10.1038/s41598-018-37341-9 |2 doi | |
| 024 | 7 | |a 30432741 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a angol | ||
| 100 | 1 | |a Gajdos Tamás | |
| 245 | 1 | 0 | |a mmSTORM |h [elektronikus dokumentum] : |b Multimodal localization based super-resolution microscopy / |c Gajdos Tamás |
| 260 | |c 2019 | ||
| 300 | |a Paper 798-9 p | ||
| 490 | 0 | |a SCIENTIFIC REPORTS |v 9 | |
| 520 | 3 | |a Super-resolution localization microscopy provides a powerful tool to study biochemical mechanisms at single molecule level. Although the lateral position of the fluorescent dye molecules can be determined routinely with high precision, measurement of other modalities such as 3D and multicolor without the degradation of the original super-resolved image is still in the focus. In this paper a dual-objective multimodal single molecule localization microscopy (SMLM) technique has been developed, optimized and tested. The proposed optical arrangement can be implemented onto a conventional inverted microscope without serious system modification. The performance of the method was tested using fluorescence beads, F-actin filaments and sarcomere structures. It was shown that the proposed imaging method does not degrade the image quality of the original SMLM 2D image but could provide information on the axial position or emission spectra of the dye molecules. | |
| 700 | 0 | 1 | |a Cserteg Zsófia |e aut |
| 700 | 0 | 1 | |a Szikora Szilárd |e aut |
| 700 | 0 | 1 | |a Novák Tibor |e aut |
| 700 | 0 | 1 | |a Kovács Bálint Barna H. |e aut |
| 700 | 0 | 1 | |a Szabó Gábor |e aut |
| 700 | 0 | 1 | |a Mihály József |e aut |
| 700 | 0 | 1 | |a Erdélyi Miklós |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/15994/1/s41598-018-37341-9.pdf |z Dokumentum-elérés |