Third-generation sequencing reveals extensive polycistronism and transcriptional overlapping in a baculovirus
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an insect-pathogen baculovirus. In this study, we applied the Oxford Nanopore Technologies platform for the analysis of the polyadenylated fraction of the viral transcriptome using both cDNA and direct RNA sequencing methods. We id...
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| Dokumentumtípus: | Cikk |
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Nature Publishing Group
2018
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| Sorozat: | SCIENTIFIC REPORTS
8 No. 1 |
| doi: | 10.1038/s41598-018-26955-8 |
| mtmt: | 3383352 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/13461 |
| LEADER | 02159nab a2200289 i 4500 | ||
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| 024 | 7 | |a 10.1038/s41598-018-26955-8 |2 doi | |
| 024 | 7 | |a 3383352 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
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| 100 | 1 | |a Moldován Norbert | |
| 245 | 1 | 0 | |a Third-generation sequencing reveals extensive polycistronism and transcriptional overlapping in a baculovirus |h [elektronikus dokumentum] / |c Moldován Norbert |
| 260 | |a Nature Publishing Group |c 2018 | ||
| 300 | |a Terjedelem: 11 p.-Azonosító: 8604 | ||
| 490 | 0 | |a SCIENTIFIC REPORTS |v 8 No. 1 | |
| 520 | 3 | |a The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an insect-pathogen baculovirus. In this study, we applied the Oxford Nanopore Technologies platform for the analysis of the polyadenylated fraction of the viral transcriptome using both cDNA and direct RNA sequencing methods. We identified and annotated altogether 132 novel transcripts and transcript isoforms, including 4 coding and 4 non-coding RNA molecules, 47 length variants, 5 splice isoforms, as well as 23 polycistronic and 49 complex transcripts. All of the identified novel protein-coding genes were 5'-truncated forms of longer host genes. In this work, we demonstrated that in the case of transcript start site isoforms, the promoters and the initiator sequence of the longer and shorter variants belong to the same kinetic class. Long-read sequencing also revealed a complex meshwork of transcriptional overlaps, the function of which needs to be clarified. Additionally, we developed bioinformatics methods to improve the transcript annotation and to eliminate the non-specific transcription reads generated by template switching and false priming. | |
| 700 | 0 | 1 | |a Tombácz Dóra |e aut |
| 700 | 0 | 1 | |a Szűcs Attila |e aut |
| 700 | 0 | 1 | |a Csabai Zsolt |e aut |
| 700 | 0 | 1 | |a Balázs Zsolt |e aut |
| 700 | 0 | 1 | |a Kis Emese |e aut |
| 700 | 0 | 1 | |a Molnár Judit |e aut |
| 700 | 0 | 1 | |a Boldogkői Zsolt |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/13461/1/s41598_018_26955_8_u.pdf |z Dokumentum-elérés |