Multivalent foldamer-based affinity assay for selective recognition of Aβ oligomers
Abstract Mimicking the molecular recognition functionality of antibodies is a great challenge. Foldamers are attractive candidates because of their relatively small size and designable interaction surface. This paper describes a sandwich type enzyme-linked immunoassay with a tetravalent β-peptide fo...
Elmentve itt :
| Szerzők: | |
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2017
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| Sorozat: | ANALYTICA CHIMICA ACTA
960 |
| doi: | 10.1016/j.aca.2017.01.013 |
| mtmt: | 3193785 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/11875 |
| LEADER | 01747nab a2200289 i 4500 | ||
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| 001 | publ11875 | ||
| 005 | 20191114170909.0 | ||
| 008 | 170830s2017 hu o 0|| zxx d | ||
| 022 | |a 0003-2670 | ||
| 024 | 7 | |a 10.1016/j.aca.2017.01.013 |2 doi | |
| 024 | 7 | |a 3193785 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a zxx | ||
| 100 | 1 | |a Olajos Gábor | |
| 245 | 1 | 0 | |a Multivalent foldamer-based affinity assay for selective recognition of Aβ oligomers |h [elektronikus dokumentum] / |c Olajos Gábor |
| 260 | |c 2017 | ||
| 300 | |a 131-137 | ||
| 490 | 0 | |a ANALYTICA CHIMICA ACTA |v 960 | |
| 520 | 3 | |a Abstract Mimicking the molecular recognition functionality of antibodies is a great challenge. Foldamers are attractive candidates because of their relatively small size and designable interaction surface. This paper describes a sandwich type enzyme-linked immunoassay with a tetravalent β-peptide foldamer helix array as capture element and enzyme labeled tracer antibodies. The assay was found to be selective to β-amyloid oligomeric species with surface features transiently present in ongoing aggregation. In optimized conditions, with special emphasis on the foldamer immobilization, a detection limit of 5 pM was achieved with a linear range of 10–500 pM. These results suggest that protein mimetic foldamers can be useful tools in biosensors and affinity assays. | |
| 700 | 0 | 1 | |a Bartus Éva |e aut |
| 700 | 0 | 1 | |a Schuster Ildikó |e aut |
| 700 | 0 | 1 | |a Lautner Gergely |e aut |
| 700 | 0 | 1 | |a Gyurcsányi Ervin Róbert |e aut |
| 700 | 0 | 1 | |a Szögi Titanilla |e aut |
| 700 | 0 | 1 | |a Fülöp Lívia |e aut |
| 700 | 0 | 1 | |a Martinek Tamás A. |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/11875/1/1_s2.0_S0003267017300922_main_u.pdf |z Dokumentum-elérés |