Effect of sodium nitrite on ischaemia and reperfusion-induced arrhythmias in anaesthetized dogs is protein s-nitrosylation involved? /
BACKGROUND AND PURPOSE: To provide evidence for the protective role of inorganic nitrite against acute ischaemia and reperfusion-induced ventricular arrhythmias in a large animal model. EXPERIMENTAL APPROACH: Dogs, anaesthetized with chloralose and urethane, were administered intravenously with sod...
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| Dokumentumtípus: | Cikk |
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Public Library of Science (PLoS)
2015
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| Sorozat: | PLOS ONE
10 No. 4 |
| doi: | 10.1371/journal.pone.0122243 |
| mtmt: | 2888324 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/10828 |
| LEADER | 02991nab a2200301 i 4500 | ||
|---|---|---|---|
| 001 | publ10828 | ||
| 005 | 20190716082603.0 | ||
| 008 | 170227s2015 hu o 0|| angol d | ||
| 022 | |a 1932-6203 | ||
| 024 | 7 | |a 10.1371/journal.pone.0122243 |2 doi | |
| 024 | 7 | |a 2888324 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a angol | ||
| 100 | 1 | |a Kovács Mária | |
| 245 | 1 | 0 | |a Effect of sodium nitrite on ischaemia and reperfusion-induced arrhythmias in anaesthetized dogs |h [elektronikus dokumentum] : |b is protein s-nitrosylation involved? / |c Kovács Mária |
| 260 | |a Public Library of Science (PLoS) |c 2015 | ||
| 300 | |a Terjedelem: 22 p.-Azonosító: e0122243 | ||
| 490 | 0 | |a PLOS ONE |v 10 No. 4 | |
| 520 | 3 | |a BACKGROUND AND PURPOSE: To provide evidence for the protective role of inorganic nitrite against acute ischaemia and reperfusion-induced ventricular arrhythmias in a large animal model. EXPERIMENTAL APPROACH: Dogs, anaesthetized with chloralose and urethane, were administered intravenously with sodium nitrite (0.2 micromolkg-1min-1) in two protocols. In protocol 1 nitrite was infused 10 min prior to and during a 25 min occlusion of the left anterior descending (LAD) coronary artery (NaNO2-PO; n = 14), whereas in protocol 2 the infusion was started 10 min prior to reperfusion of the occluded vessel (NaNO2-PR; n = 12). Control dogs (n = 15) were infused with saline and subjected to the same period of ischaemia and reperfusion. Severities of ischaemia and ventricular arrhythmias, as well as changes in plasma nitrate/nitrite (NOx) levels in the coronary sinus blood, were assessed throughout the experiment. Myocardial superoxide and nitrotyrosine (NT) levels were determined during reperfusion. Changes in protein S-nitrosylation (SNO) and S-glutathionylation were also examined. KEY RESULTS: Compared with controls, sodium nitrite administered either pre-occlusion or pre-reperfusion markedly suppressed the number and severity of ventricular arrhythmias during occlusion and increased survival (0% vs. 50 and 92%) upon reperfusion. There were also significant decreases in superoxide and NT levels in the nitrite treated dogs. Compared with controls, increased SNO was found only in NaNO2-PR dogs, whereas S-glutathionylation occurred primarily in NaNO2-PO dogs. CONCLUSIONS: Intravenous infusion of nitrite profoundly reduced the severity of ventricular arrhythmias resulting from acute ischaemia and reperfusion in anaesthetized dogs. This effect, among several others, may result from an NO-mediated reduction in oxidative stress, perhaps through protein SNO and/or S-glutathionylation. | |
| 700 | 0 | 1 | |a Kiss Attila |e aut |
| 700 | 0 | 1 | |a Gönczi Márton |e aut |
| 700 | 0 | 1 | |a Miskolczi Gottfried |e aut |
| 700 | 0 | 1 | |a Seprényi György |e aut |
| 700 | 0 | 1 | |a Kaszaki József |e aut |
| 700 | 0 | 1 | |a Kohr Mark J. |e aut |
| 700 | 0 | 1 | |a Murphy Elizabeth |e aut |
| 700 | 0 | 1 | |a Végh Ágnes |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/10828/1/plosone.pdf |z Dokumentum-elérés |