Phenotypic characteristics of environmental Pseudomonas aeruginosa an in vitro study on epidemiological aspects /
Pseudomonas aeruginosa is one of the most commonly isolated species among non-fermenting Gram-negative bacteria, both from clinical samples and from environmental sources. The survival of the species in harsh conditions is ensured by the production of a protective biofilm; assessment of biofilm-form...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
University of Szeged
Szeged
2023
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| Sorozat: | Acta biologica Szegediensis
67 No. 1 |
| Kulcsszavak: | Mikrobiológia, Fertőző betegségek, Gyógyszerrezisztencia - mikrobák, Antibiotikum-ellenállás, Bakteriológia |
| Tárgyszavak: | |
| doi: | 10.14232/abs.2023.1.35-44 |
| Online Access: | http://acta.bibl.u-szeged.hu/86936 |
| LEADER | 03904nab a2200385 i 4500 | ||
|---|---|---|---|
| 001 | acta86936 | ||
| 005 | 20250415085047.0 | ||
| 008 | 250415s2023 hu o 000 eng d | ||
| 022 | |a 1588-4082 | ||
| 024 | 7 | |a 10.14232/abs.2023.1.35-44 |2 doi | |
| 040 | |a SZTE Egyetemi Kiadványok Repozitórium |b hun | ||
| 041 | |a eng | ||
| 100 | 1 | |a Gajdács Márió | |
| 245 | 1 | 0 | |a Phenotypic characteristics of environmental Pseudomonas aeruginosa |h [elektronikus dokumentum] : |b an in vitro study on epidemiological aspects / |c Gajdács Márió |
| 260 | |a University of Szeged |b Szeged |c 2023 | ||
| 300 | |a 35-44 | ||
| 490 | 0 | |a Acta biologica Szegediensis |v 67 No. 1 | |
| 520 | 3 | |a Pseudomonas aeruginosa is one of the most commonly isolated species among non-fermenting Gram-negative bacteria, both from clinical samples and from environmental sources. The survival of the species in harsh conditions is ensured by the production of a protective biofilm; assessment of biofilm-forming capacity aids future pathogen eradication strategies. The aim of our present study was to assess the relationship between antibiotic resistance, biofilm-forming capacity and other phenotypic virulence factors in environmental P. aeruginosa isolates. One hundred and fourteen (n = 114) isolates were included in the study, which were obtained from various geographical regions and environmental origins. Antimicrobial susceptibility testing was carried out using standard protocols. Biofilm-forming capacity and pyocyanin pigment production were tested using microtiter plate-based methods. Swarming, swimming and twitching motility, and siderophore-production were assessed using agar-plate based methodologies. Resistance in environmental isolates were highest for levofloxacin/ciprofloxacin 49.12% (n = 56), ceftazidime 42.98% (n = 49) and cefepime 35.96% (n = 41), while lowest for colistin 0% (n = 0); overexpression of RND-type efflux pumps was seen in 33.33% (n = 33) of isolates. 21.93% (n = 25) met the criteria to be classified as multidrug resistant (MDR). 17.54% (n = 20) of isolates were weak/non-biofilm producers, while (25.45%, n = 29) and (57.01%, n = 65) were moderate and strong biofilm producers, respectively. No significant differences were noted in biofilm-formation (OD570 values non-MDR [mean ± SD]: 0.396 ± 0.138 vs. MDR: 0.348 ± 0.181; p > 0.05) or pyocyanin pigment production (OD686 values non-MDR: 0.403 ± 0.169 vs. MDR: 0.484 ± 0.125; p > 0.05) between MDR and non-MDR environmental P. aeruginosa. Highest motility values were observed for swarming motility, followed by swimming and twitching motility; no relevant differences (p > 0.05) in motility were noted in the context of MDR status or biofilm-formation in the tested isolates. P. aeruginosa is an opportunistic pathogen with high medical importance, being a causative agent of recalcitrant infections, which are becoming difficult to treat with the onset of MDR. Further studies are warranted to assess biofilm-forming capacity, and to provide insights into the mechanisms underlying biofilm-formation both in isolates of clinical and environmental origins. | |
| 650 | 4 | |a Természettudományok | |
| 650 | 4 | |a Biológiai tudományok | |
| 650 | 4 | |a Orvos- és egészségtudomány | |
| 650 | 4 | |a Általános orvostudomány | |
| 650 | 4 | |a Egészségtudományok | |
| 695 | |a Mikrobiológia, Fertőző betegségek, Gyógyszerrezisztencia - mikrobák, Antibiotikum-ellenállás, Bakteriológia | ||
| 700 | 0 | 1 | |a Urbán Edit |e aut |
| 700 | 0 | 1 | |a Pallós Péter |e aut |
| 700 | 0 | 1 | |a Márta Adrienn |e aut |
| 700 | 0 | 1 | |a Matusovits Danica |e aut |
| 700 | 0 | 1 | |a Kárpáti Krisztina |e aut |
| 700 | 0 | 1 | |a Battah Basem |e aut |
| 700 | 0 | 1 | |a Hetta Helal F. |e aut |
| 700 | 0 | 1 | |a Khusro Ameer |e aut |
| 700 | 0 | 1 | |a Dordevic Dani |e aut |
| 700 | 0 | 1 | |a Kushkevych Ivan |e aut |
| 856 | 4 | 0 | |u http://acta.bibl.u-szeged.hu/86936/1/biologica_067_numb_001_035-044.pdf |z Dokumentum-elérés |