Insights on carbapenem-resistant Pseudomonas aeruginosa phenotypic characterization of relevant isolates /
Pseudomonas aeruginosa (P. aeruginosa) is ubiquitous in nature, and may be a causative agent in severe, life-threatening infections. In >60% of cases, β-lactam antibiotics are used in the therapy of P. aeruginosa infections, therefore the emergence of carbapenem-resistant P. aeruginosa (CRPA) is...
Elmentve itt :
| Szerzők: | |
|---|---|
| Dokumentumtípus: | Cikk |
| Megjelent: |
University of Szeged
Szeged
2021
|
| Sorozat: | Acta biologica Szegediensis
65 No. 1 |
| Kulcsszavak: | Mikrobiológia |
| Tárgyszavak: | |
| doi: | 10.14232/abs.2021.1.105-112 |
| Online Access: | http://acta.bibl.u-szeged.hu/75585 |
| LEADER | 02784nab a2200277 i 4500 | ||
|---|---|---|---|
| 001 | acta75585 | ||
| 005 | 20220512095912.0 | ||
| 008 | 220512s2021 hu o 0|| eng d | ||
| 022 | |a 1588-4082 | ||
| 024 | 7 | |a 10.14232/abs.2021.1.105-112 |2 doi | |
| 040 | |a SZTE Egyetemi Kiadványok Repozitórium |b hun | ||
| 041 | |a eng | ||
| 100 | 1 | |a Gajdács Márió | |
| 245 | 1 | 0 | |a Insights on carbapenem-resistant Pseudomonas aeruginosa |h [elektronikus dokumentum] : |b phenotypic characterization of relevant isolates / |c Gajdács Márió |
| 260 | |a University of Szeged |b Szeged |c 2021 | ||
| 300 | |a 105-112 | ||
| 490 | 0 | |a Acta biologica Szegediensis |v 65 No. 1 | |
| 520 | 3 | |a Pseudomonas aeruginosa (P. aeruginosa) is ubiquitous in nature, and may be a causative agent in severe, life-threatening infections. In >60% of cases, β-lactam antibiotics are used in the therapy of P. aeruginosa infections, therefore the emergence of carbapenem-resistant P. aeruginosa (CRPA) is a significant clinical concern. In this study, phenotypic methods were used to characterize fifty-four (n = 54) P. aeruginosa isolates, which were included based on their suspected non-susceptibility to meropenem. Minimum inhibitory concentrations (MICs) of meropenem, ceftazidime, cefepime, ciprofloxacin, gentamicin, were determined using E-tests, while colistin MICs were determined using broth microdilution. The isolates were subjected to the modified Hodge test (MHT), the modified carbapenem-inactivation method (mCIM) and the imipenem/EDTA combined disk test (CDT). AmpC and efflux pump overexpression was studied using agar plates containing cloxacillin and phenylalanine-arginine β-naphthylamide (PAβN), respectively. Assessment of biofilm-formation was carried out using the crystal violet tube-adherence method. 38.9% of the strains showed meropenem MICs in the resistant range (>8 mg/L). Efflux-pump overexpression and AmpC-hyperproduction was seen in 44.4% and 35.2% of isolates, respectively. 88.8% of the isolates were characterized as strong biofilm-producers. On the other hand, the presence of carbapenemases was suspected in a minority (16.7%) of tested isolates. As safe and effective therapeutic options in carbapenem-resistant Gram-negative infections are severely limited, characterization of these isolates using phenotypic and molecular-based methods is important to provide insights into the epidemiological features of these pathogens. | |
| 650 | 4 | |a Természettudományok | |
| 650 | 4 | |a Biológiai tudományok | |
| 695 | |a Mikrobiológia | ||
| 700 | 0 | 1 | |a Kárpáti Krisztina |e aut |
| 700 | 0 | 1 | |a Stájer Anette |e aut |
| 700 | 0 | 1 | |a Zanetti Stefania |e aut |
| 700 | 0 | 1 | |a Donadu Matthew Gavino |e aut |
| 856 | 4 | 0 | |u http://acta.bibl.u-szeged.hu/75585/1/biologica_065_numb_001_105-112.pdf |z Dokumentum-elérés |