TU-DAMD employment for molecular characterization of Salvia judaica and Salvia palaestina species

Genetic diversity in perennial Salvia judaica Boiss (Judean sage) and Salvia palaestina Benth (Palestinian sage) species using touch-up directed amplifi cation of minisatellite region DNA (TU-DAMD) has been performed in two separated sets; in the fi rst set (set A) the initial annealing temperature...

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Bibliographic Details
Main Author: Saleh Basel
Format: Article
Published: University of Szeged Szeged 2021
Series:Acta biologica Szegediensis 65 No. 1
Kulcsszavak:Biológia - molekuláris, Növénygenetika
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doi:10.14232/abs.2021.1.11-16

Online Access:http://acta.bibl.u-szeged.hu/75575
Description
Summary:Genetic diversity in perennial Salvia judaica Boiss (Judean sage) and Salvia palaestina Benth (Palestinian sage) species using touch-up directed amplifi cation of minisatellite region DNA (TU-DAMD) has been performed in two separated sets; in the fi rst set (set A) the initial annealing temperature was increased from 50 °C to 55 °C, whereas, in the second one (set B), it increased from 55 °C to 60 °C by 0.5 °C/cycle during the fi rst 10 PCR amplifi cation cycles. Fifteen DAMD primers have been tested for each set. Set (A) produced 89.39% polymorphism level (P%) with polymorphic information content (PIC) average of 0.33 and marker index (MI) average of 3.96. Whereas, in set (B) these values were recorded to be 94.02%, 0.34 and 3.98 for P%, PIC and MI, respectively. Data showed that the two mentioned sets successfully highlighted high polymorphism level between the two studied Salvia sp. This work studies genetic diversity of S. judaica and S. palaestina species using TU-DAMD test as a novel molecular marker.
Physical Description:11-16
ISSN:1588-4082