Expression and production optimization of the cationic antimicrobial peptide indolicidin by the recombinant E. coli C41 (DE3) clones /
The cytoplasmic granules of bovine neutrophils naturally possess indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask level production and expression optimizations of the indol...
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| Dokumentumtípus: | Cikk |
| Megjelent: |
University of Szeged
Szeged
2018
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| Sorozat: | Acta biologica Szegediensis
62 No. 1 |
| Kulcsszavak: | Mikrobiológia |
| doi: | 10.14232/abs.2018.1.61-66 |
| Online Access: | http://acta.bibl.u-szeged.hu/58199 |
| LEADER | 01848nab a2200289 i 4500 | ||
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| 001 | acta58199 | ||
| 005 | 20210412140655.0 | ||
| 008 | 191121s2018 hu o 0|| zxx d | ||
| 022 | |a 1588-4082 | ||
| 024 | 7 | |a 10.14232/abs.2018.1.61-66 |2 doi | |
| 040 | |a SZTE Egyetemi Kiadványok Repozitórium |b hun | ||
| 041 | |a zxx | ||
| 100 | 1 | |a Selvam Kanesan Panneer | |
| 245 | 1 | 0 | |a Expression and production optimization of the cationic antimicrobial peptide |h [elektronikus dokumentum] : |b indolicidin by the recombinant E. coli C41 (DE3) clones / |c Selvam Kanesan Panneer |
| 260 | |a University of Szeged |b Szeged |c 2018 | ||
| 300 | |a 61-66 | ||
| 490 | 0 | |a Acta biologica Szegediensis |v 62 No. 1 | |
| 520 | 3 | |a The cytoplasmic granules of bovine neutrophils naturally possess indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask level production and expression optimizations of the indolicidin by the recombinant Escherichia coli C41 (DE3) clones (transformed with pET21a(+) plasmid carrying indolicidin gene) were carried out under standard conditions, as to determine the conditions required for maximal production. It was determined that a concentration of 1 mM of IPTG was effective, the 2×YT with salts and LB media at pH 7.5 with 3-6 h of incubation were required for maximal indolicidin expression. | |
| 695 | |a Mikrobiológia | ||
| 700 | 0 | 1 | |a Nambirajan Guruvu |e aut |
| 700 | 0 | 1 | |a Annamalai Balasubramaniam |e aut |
| 700 | 0 | 1 | |a Alaidarous Mohammed |e aut |
| 700 | 0 | 1 | |a Alshehri Bader Mohammed |e aut |
| 700 | 0 | 2 | |a Bin Dukhyil Abdul Aziz A. |e aut |
| 700 | 0 | 2 | |a Shobana Coimbatore Subramanian |e aut |
| 700 | 0 | 2 | |a Manikandan Palanisamy |e aut |
| 856 | 4 | 0 | |u http://acta.bibl.u-szeged.hu/58199/1/biologica_062_numb_001_061-066.pdf |z Dokumentum-elérés |