Expression and production optimization of the cationic antimicrobial peptide indolicidin by the recombinant E. coli C41 (DE3) clones /

The cytoplasmic granules of bovine neutrophils naturally possess indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask level production and expression optimizations of the indol...

Teljes leírás

Elmentve itt :
Bibliográfiai részletek
Szerzők: Selvam Kanesan Panneer
Nambirajan Guruvu
Annamalai Balasubramaniam
Alaidarous Mohammed
Alshehri Bader Mohammed
Bin Dukhyil Abdul Aziz A.
Shobana Coimbatore Subramanian
Manikandan Palanisamy
Dokumentumtípus: Cikk
Megjelent: University of Szeged Szeged 2018
Sorozat:Acta biologica Szegediensis 62 No. 1
Kulcsszavak:Mikrobiológia
doi:10.14232/abs.2018.1.61-66

Online Access:http://acta.bibl.u-szeged.hu/58199
Leíró adatok
Tartalmi kivonat:The cytoplasmic granules of bovine neutrophils naturally possess indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask level production and expression optimizations of the indolicidin by the recombinant Escherichia coli C41 (DE3) clones (transformed with pET21a(+) plasmid carrying indolicidin gene) were carried out under standard conditions, as to determine the conditions required for maximal production. It was determined that a concentration of 1 mM of IPTG was effective, the 2×YT with salts and LB media at pH 7.5 with 3-6 h of incubation were required for maximal indolicidin expression.
Terjedelem/Fizikai jellemzők:61-66
ISSN:1588-4082