Concomitant production of delta-endotoxins and proteases of Bacillus thuringiensis subsp. kurstaki in a low-cost medium effect of medium components /
Bacillus thuringiensis is a bacterium, commonly used as a biological pesticide, and produces entomotoxic parasporal crystals (delta-endotoxins) and photolytic enzymes involved in several biological processes. The aim of present work was to enhance the production of delta-endotoxins by an isolated Ba...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2013
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| Sorozat: | Acta biologica Szegediensis
57 No. 1 |
| Kulcsszavak: | Természettudomány, Biológia |
| Online Access: | http://acta.bibl.u-szeged.hu/31277 |
| LEADER | 02219nab a2200193 i 4500 | ||
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| 001 | acta31277 | ||
| 005 | 20210412155223.0 | ||
| 008 | 161017s2013 hu o 0|| eng d | ||
| 022 | |a 1588-385X | ||
| 040 | |a SZTE Egyetemi Kiadványok Repozitórium |b hun | ||
| 041 | |a eng | ||
| 100 | 1 | |a Ennouri Karim | |
| 245 | 1 | 0 | |a Concomitant production of delta-endotoxins and proteases of Bacillus thuringiensis subsp. kurstaki in a low-cost medium |h [elektronikus dokumentum] : |b effect of medium components / |c Ennouri Karim |
| 260 | |c 2013 | ||
| 300 | |a 13-19 | ||
| 490 | 0 | |a Acta biologica Szegediensis |v 57 No. 1 | |
| 520 | 3 | |a Bacillus thuringiensis is a bacterium, commonly used as a biological pesticide, and produces entomotoxic parasporal crystals (delta-endotoxins) and photolytic enzymes involved in several biological processes. The aim of present work was to enhance the production of delta-endotoxins by an isolated Bacillus thuringiensis subsp. kurstaki strain. The adopted approach was based on studying both delta-endotoxins and proteolytic activities production. Trials were carried out based on the Plackett-Burman experimental design. The statistical analysis revealed that the soybean meal was the main significant ingredient (confidence level= 99.5%) for both delta-endotoxins and proteolytic activities productions. Starch and FeSO4 were considered as significant ingredients for only protease production, the confidence levels were 98.8% and 97% respectively. The study of the nutrients effects brought out that K2HPO4, FeSO4 and starch, exhibit an opposite effect on the synthesis of delta-endotoxins and proteolytic activity production. On the other hand, MnSO4, MgSO4 and soybean meal are considered as the ingredients having dual positive effect on proteolytic activities and delta-endotoxin productions, whereas KH2PO4 had an inhibitory effect. The optimisation strategy using mathematical methods offers an efficient technique to optimize media leading to the increase delta-endotoxin production by Bacillus thuringiensis subsp. kurstaki strains by clustering of the effects of medium components. | |
| 695 | |a Természettudomány, Biológia | ||
| 856 | 4 | 0 | |u http://acta.bibl.u-szeged.hu/31277/1/5713.pdf |z Dokumentum-elérés |